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Literature summary for 1.1.1.85 extracted from

  • Sekiguchi, T.; Suda, M.; Sekiguchi, T.; Nosoh, Y.
    Cloning and DNA homology of 3-isopropylmalate dehydrogenase genes from thermophilic bacilli (1986), FEMS Microbiol. Lett., 36, 41-45.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli using pBR322 as a vector [Bacillus] caldotenax
expression in Escherichia coli using pBR322 as a vector [Bacillus] caldolyticus

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Escherichia coli
-
-
-
Saccharomyces cerevisiae
-
-
-
Thermus thermophilus
-
-
-
[Bacillus] caldolyticus
-
-
-
[Bacillus] caldotenax
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+
-
Bacillus subtilis 2-oxoisocaproate + NADH + H+ + CO2
-
?
(2R,3S)-3-isopropylmalate + NAD+
-
Thermus thermophilus 2-oxoisocaproate + NADH + H+ + CO2
-
?
(2R,3S)-3-isopropylmalate + NAD+
-
Escherichia coli 2-oxoisocaproate + NADH + H+ + CO2
-
?
(2R,3S)-3-isopropylmalate + NAD+
-
Saccharomyces cerevisiae 2-oxoisocaproate + NADH + H+ + CO2
-
?
(2R,3S)-3-isopropylmalate + NAD+
-
[Bacillus] caldotenax 2-oxoisocaproate + NADH + H+ + CO2
-
?
(2R,3S)-3-isopropylmalate + NAD+
-
[Bacillus] caldolyticus 2-oxoisocaproate + NADH + H+ + CO2
-
?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bacillus subtilis
NAD+
-
Thermus thermophilus
NAD+
-
Escherichia coli
NAD+
-
Saccharomyces cerevisiae
NAD+
-
[Bacillus] caldotenax
NAD+
-
[Bacillus] caldolyticus