Literature summary for 1.1.1.85 extracted from

Onodera, K.; Moriyama, H.; Takenaka, A.; Tanaka, N.; Akutsu, N.; Muro, M.; Oshima, T.; Imada, K.; Sato, M.; Katsube, Y.
Crystallization and preliminary X-ray studies of a Bacillus subtilis and Thermus thermophilus HB8 chimeric 3-isopropylmalate dehydrogenase (1991), J. Biochem., 109, 1-2.

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Cloned(Commentary)

Cloned (Comment)	Organism
construction of a chimeric gene by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, expression in Escherichia coli	Bacillus subtilis
construction of a chimeric gene by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, expression in Escherichia coli	Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, chimeric enzyme constructed by fusing the gene of Bacillus subtilis and Thermus thermophilus coding for 3-isopropylmalate dehydrogenase, expression in Escherichia coli	Bacillus subtilis
hanging drop vapor diffusion method, chimeric enzyme constructed by fusing the gene of Bacillus subtilis and Thermus thermophilus coding for 3-isopropylmalate dehydrogenase, expression in Escherichia coli	Thermus thermophilus

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	-	-
Thermus thermophilus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2R,3S)-3-isopropylmalate + NAD+	-	Bacillus subtilis	2-oxoisocaproate + NADH + H+ + CO2	-	?
(2R,3S)-3-isopropylmalate + NAD+	-	Thermus thermophilus	2-oxoisocaproate + NADH + H+ + CO2	-	?

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Bacillus subtilis
NAD+	-	Thermus thermophilus

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Release 2023.1 (January 2023)