Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | optimal activity in presence of both Mn2+ and NH4+ | Cereibacter sphaeroides |
General Stability | Organism |
---|---|
10-40% loss of activity during repeated freezing and thawing | Cereibacter sphaeroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Cereibacter sphaeroides | |
Dihydroxyfumarate | noncompetitive | Cereibacter sphaeroides | |
iodoacetate | - |
Cereibacter sphaeroides | |
L-Tartrate | competitive with respect to D-malate | Cereibacter sphaeroides | |
meso-tartrate | competitive | Cereibacter sphaeroides | |
oxaloacetate | competitive | Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
NAD+ | - |
Cereibacter sphaeroides | |
0.17 | - |
D-malate | - |
Cereibacter sphaeroides |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 30% of the activity with Mn2+ | Cereibacter sphaeroides | |
K2SO4 | 50 mM, 56% of the activity with Mn2+ | Cereibacter sphaeroides | |
KCl | 50 mM, 63% of the activity with Mn2+ | Cereibacter sphaeroides | |
Mg2+ | 5 mM, 59% of the activity with Mn2+ | Cereibacter sphaeroides | |
Mn2+ | Km: 0.016 mM, optimal activity in presence of both Mn2+ and NH4+ | Cereibacter sphaeroides | |
NaCl | 50 mM, 19% of the activity with Mn2+ | Cereibacter sphaeroides | |
Zn2+ | 0.1 mM, 14% of the activity with Mn2+ | Cereibacter sphaeroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38500 | - |
4 * 38500, SDS-PAGE | Cereibacter sphaeroides |
158000 | - |
gel filtration | Cereibacter sphaeroides |
162000 | - |
equilibrium sedimentation | Cereibacter sphaeroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
- |
- |
Cereibacter sphaeroides Y | - |
- |
- |
Purification (Comment) | Organism |
---|---|
bifunctional L-(+)-tartrate-D-(+)-malate dehydrogenase | Cereibacter sphaeroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.6 | - |
- |
Cereibacter sphaeroides |
Storage Stability | Organism |
---|---|
-20°C, stable for months | Cereibacter sphaeroides |
0°C, stable for 30 h, even at protein concentration below 1 mg/ml | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-malate + NAD+ | - |
Cereibacter sphaeroides | pyruvate + CO2 + NADH | - |
? | |
(R)-malate + NAD+ | - |
Cereibacter sphaeroides Y | pyruvate + CO2 + NADH | - |
? | |
additional information | bifunctional L(+)-tartrate dehydrogenase (decarboxylating) EC 1.1.1.93/EC 1.1.1.83 also catalyzes nonoxidative decarboxylation of L-tartrate | Cereibacter sphaeroides | ? | - |
? | |
additional information | bifunctional L(+)-tartrate dehydrogenase (decarboxylating) EC 1.1.1.93/EC 1.1.1.83 also catalyzes nonoxidative decarboxylation of L-tartrate | Cereibacter sphaeroides Y | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 38500, SDS-PAGE | Cereibacter sphaeroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Cereibacter sphaeroides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
half-life: 30 h | Cereibacter sphaeroides |
50 | - |
half-life: 120 min | Cereibacter sphaeroides |
55 | - |
half-life: 30 min | Cereibacter sphaeroides |
60 | - |
half-life: 9 min | Cereibacter sphaeroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | 9 | - |
Cereibacter sphaeroides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10.6 | at pH 7.0 and 10.6: about 50% of activity maximum | Cereibacter sphaeroides |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
30°C, 4 h, stable | Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Cereibacter sphaeroides |