Activating Compound | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | enzyme is activated by reduction of its N-terminal and C-terminal disulfides by reduced thioredoxin. The activation is inhibited by NADP+ | Sorghum sp. |
Protein Variants | Comment | Organism |
---|---|---|
G84D | 10fold lower Km for NADH, Km for NADPH remains unchanged | Sorghum sp. |
G84D/S851I/R87Q/S88A | changed cofactor specificity from NADPH to NADH, the activation of the NAD-specific thiol-regulated enzyme is inhibited by NAD+ but no longer by NADP+ | Sorghum sp. |
S85I/R87Q/S88A | 7fold increase in the Km-value for NADPH and 4fold decrease in Km-value for NADH | Sorghum sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Sorghum sp. | 9507 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sorghum sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADPH | - |
Sorghum sp. | (S)-malate + NADP+ | - |
? |