Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetone | increases the activity of the oxidized truncated enzyme form, optimal concentration 15% | Pisum sativum | |
carboxypeptidase Y | incubation of the native oxidized enzyme at pH 6.0, 25°C, results in slow activation. A small carboxy-terminal peptide of the native enzyme is accessible to proteolytic degradation followed by activation of the inactive oxidized enzyme. This peptide is involved in the regulation of activity, tetramer formation and thioredoxin binding | Pisum sativum | |
dithiothreitol | activates | Pisum sativum | |
methanol | increases the activity of the oxidized truncated enzyme form, optimal concentration 8% | Pisum sativum |
General Stability | Organism |
---|---|
a small carboxy-terminal peptide of the native enzyme is accessible to proteolytic degradation followed by activation of the inactive oxidized enzyme. This peptide is involved in the regulation of activity, tetramer formation and thioredoxin binding | Pisum sativum |
denaturation by 2 M guanidine-HCl | Pisum sativum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | oxidized truncated enzyme | Pisum sativum | |
oxaloacetate | pH 8, substrate inhibition of the reduced truncated enzyme | Pisum sativum | |
Urea | oxidized truncated enzyme | Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.039 | - |
NADPH | reduced native enzyme | Pisum sativum | |
0.042 | - |
oxaloacetate | reduced truncated enzyme | Pisum sativum | |
0.046 | - |
NADPH | oxidized truncated enzyme | Pisum sativum | |
0.048 | - |
oxaloacetate | reduced native enzyme | Pisum sativum | |
0.089 | - |
NADPH | reduced truncated enzyme | Pisum sativum | |
1.2 | - |
oxaloacetate | oxidized truncated enzyme | Pisum sativum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Pisum sativum | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38900 | - |
4 * 38900, native enzyme, SDS-PAGE | Pisum sativum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | a small carboxy-terminal peptide of the native enzyme is accessible to proteolytic degradation followed by activation of the inactive oxidized enzyme. This peptide is involved in the regulation of activity, tetramer formation and thioredoxin binding | Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADPH | - |
Pisum sativum | (S)-malate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 38900, native enzyme, SDS-PAGE | Pisum sativum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | reduction of oxaloacetate, oxidized truncated enzyme | Pisum sativum |
8 | 8.5 | active enzyme | Pisum sativum |