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Literature summary for 1.1.1.82 extracted from

  • Vairinhos, F.; Simon, J.P.
    Purification and molecular forms of NADP+-malate dehydrogenase from two populations of the C4 weed Echinochloa crus-galli (L.) Beauv. (Poaceae) (1990), Plant Sci., 71, 173-177.
No PubMed abstract available

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40306
-
2 * 40306, enzyme from population Quebec, SDS-PAGE Echinochloa crus-galli
41177
-
2 * 41177, enzyme from population Mississippi, SDS-PAGE Echinochloa crus-galli
83500
-
enzyme from population Quebec, non-denaturing PAGE Echinochloa crus-galli
85450
-
isoenzyme 1 and 2 from Mississippi population, non-denaturing PAGE Echinochloa crus-galli
86700
-
isoenzyme 3 from Mississippi population, non-denaturing PAGE Echinochloa crus-galli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxaloacetate + NADPH Echinochloa crus-galli
-
(S)-malate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Echinochloa crus-galli
-
L. Beauv., population Quebec and population Mississippi
-

Purification (Commentary)

Purification (Comment) Organism
one protein band from the Quebec population, three isozymes from Mississippi population Echinochloa crus-galli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
181
-
enzyme from population Mississippi Echinochloa crus-galli
211
-
enzyme from population Quebec Echinochloa crus-galli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxaloacetate + NADPH
-
Echinochloa crus-galli (S)-malate + NADP+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 40306, enzyme from population Quebec, SDS-PAGE Echinochloa crus-galli
dimer 2 * 41177, enzyme from population Mississippi, SDS-PAGE Echinochloa crus-galli

Cofactor

Cofactor Comment Organism Structure
NADPH cofactor Echinochloa crus-galli