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Literature summary for 1.1.1.8 extracted from
- Comparative study of Leishmania mexicana and trypanosoma brucei NAD-dependent glycerol-3-phosphate dehydrogenase (2000), Mol. Biochem. Parasitol., 106, 83-91.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant in Escherichia coli | Leishmania mexicana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-hydroxy-1,2,3-nonadecanetricarboxylic acid | trypanocidal drug, IC50 0.00055 mM | Leishmania mexicana |  |
| 2-hydroxy-1,2,3-nonadecanetricarboxylic acid | IC50 0.0011 mM | Trypanosoma brucei | |
| dihydroxyacetone phosphate | substrate inhibition at 0.2 mM | Leishmania mexicana | |
| dihydroxyacetone phosphate | substrate inhibition at 0.2 mM | Trypanosoma brucei | |
| Melarsen oxide | active principle of the trypanocidal drug melarsoprol and cymelarsen | Leishmania mexicana | |
| Melarsen oxide | active principle of the trypanocidal drug melarsoprol and cymelarsen; cymelarsen, IC50 0.0015-0.005 mM | Trypanosoma brucei | |
| NaCl | strongly inhibits, 50% activity at 250 mM NaCl | Leishmania mexicana | |
| NaCl | strongly inhibits, 50% activity at 250 mM NaCl | Trypanosoma brucei | |
| NADPH | inhibits in presence of saturing concentration of NADH and dihydroxyacetone phosphate | Leishmania mexicana | |
| NADPH | inhibits in presence of saturing concentration of NADH and dihydroxyacetone phosphate | Trypanosoma brucei | |
| Nucleic acids | strongly inhibit | Leishmania mexicana | |
| p-chloromercuribenzoate | 0.1 mM, reversed by thiols, e.g. dithiothreitol | Trypanosoma brucei | |
| suramin | trypanocidal drug, potent inhibitor, IC50 0.0002 mM | Leishmania mexicana | |
| suramin | IC50 0.00044 mM | Trypanosoma brucei | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
NADH | - |
Trypanosoma brucei | |
| 0.024 | - |
NADH | - |
Leishmania mexicana | |
| 0.1 | - |
dihydroxyacetone phosphate | - |
Trypanosoma brucei | |
| 0.12 | - |
dihydroxyacetone phosphate | - |
Leishmania mexicana | |
| 0.32 | - |
NAD+ | - |
Trypanosoma brucei | |
| 0.42 | - |
NAD+ | - |
Leishmania mexicana | |
| 1.7 | - |
glycerol-3-phosphate | - |
Leishmania mexicana | |
| 2.25 | - |
glycerol-3-phosphate | - |
Trypanosoma brucei | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| glycosome | - |
Trypanosoma brucei | 20015 | - |
| glycosome | - |
Leishmania mexicana | 20015 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 37000, SDS-PAGE | Leishmania mexicana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sn-glycerol 3-phosphate + NAD+ | Trypanosoma brucei | - |
dihydroxyacetone phosphate + NADH + H+ | - |
? | |
| sn-glycerol 3-phosphate + NAD+ | Leishmania mexicana | - |
dihydroxyacetone phosphate + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania mexicana | - |
- |
- |
| Trypanosoma brucei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trypanosoma brucei |
- |
Leishmania mexicana |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 154 | - |
- |
Leishmania mexicana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sn-glycerol 3-phosphate + NAD+ | - |
Trypanosoma brucei | dihydroxyacetone phosphate + NADH + H+ | - |
? | |
| sn-glycerol 3-phosphate + NAD+ | - |
Leishmania mexicana | dihydroxyacetone phosphate + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, SDS-PAGE | Leishmania mexicana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
glycerol-3-phosphate formation | Trypanosoma brucei |
| 6.5 | - |
glycerol-3-phosphate formation | Leishmania mexicana |
| 9.6 | - |
dihydroxyacetone phosphate formation | Trypanosoma brucei |
| 9.6 | - |
dihydroxyacetone phosphate formation | Leishmania mexicana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.5 | highest activity, glycerol-3-phosphate formation | Trypanosoma brucei |
| 5 | 7.5 | highest activity, glycerol-3-phosphate formation | Leishmania mexicana |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | under diluted conditions | Trypanosoma brucei |
| 5 | 9 | under diluted conditions | Leishmania mexicana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Trypanosoma brucei | |
| NADH | - |
Trypanosoma brucei | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
trypanocidal drug, potent inhibitor, IC50 0.0002 mM | Leishmania mexicana | suramin | |
| 0.00044 | - |
IC50 0.00044 mM | Trypanosoma brucei | suramin | |
| 0.00055 | - |
trypanocidal drug, IC50 0.00055 mM | Leishmania mexicana | 2-hydroxy-1,2,3-nonadecanetricarboxylic acid | |
| 0.0011 | - |
IC50 0.0011 mM | Trypanosoma brucei | 2-hydroxy-1,2,3-nonadecanetricarboxylic acid | |
| 0.0015 | 0.005 | cymelarsen, IC50 0.0015-0.005 mM | Trypanosoma brucei | Melarsen oxide | |
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