Literature summary for 1.1.1.8 extracted from

Fernandez-Sousa, J.M.; Gavilanes, J.G.; Municio, A.M.; Perez-Aranda, A.
L-glycerol-3-phosphate dehydrogenase from the insect Ceratitis capitata. Purfication, physicochemical and enzymic properties (1977), Biochim. Biophys. Acta, 481, 6-24.

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General Stability

General Stability	Organism
2-mercaptoethanol stabilizes during purification	Ceratitis capitata

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	high ionic strength above 0.03 M	Ceratitis capitata

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.018	-	NADH	-	Ceratitis capitata
0.26	-	NAD+	-	Ceratitis capitata
0.33	-	dihydroxyacetone phosphate	-	Ceratitis capitata
0.74	-	sn-glycerol 3-phosphate	-	Ceratitis capitata

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33500	-	2 * 33500, SDS-PAGE	Ceratitis capitata
72500	-	69000-76000, gel filtration	Ceratitis capitata

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sn-glycerol 3-phosphate + NAD+	Ceratitis capitata	-	dihydroxyacetone phosphate + NADH + H+	-	r
sn-glycerol 3-phosphate + NAD+	Ceratitis capitata Wiedemann	-	dihydroxyacetone phosphate + NADH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Ceratitis capitata	-	mediterranean fruit fly	-
Ceratitis capitata Wiedemann	-	mediterranean fruit fly	-

Purification (Commentary)

Purification (Comment)	Organism
-	Ceratitis capitata

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
198.9	-	-	Ceratitis capitata

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sn-glycerol 3-phosphate + NAD+	-	Ceratitis capitata	dihydroxyacetone phosphate + NADH + H+	-	r
sn-glycerol 3-phosphate + NAD+	-	Ceratitis capitata Wiedemann	dihydroxyacetone phosphate + NADH + H+	-	r
sn-glycerol 3-phosphate + NAD+	-	Ceratitis capitata	glycerone phosphate + NADH + H+	-	r
sn-glycerol 3-phosphate + NAD+	-	Ceratitis capitata Wiedemann	glycerone phosphate + NADH + H+	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 33500, SDS-PAGE	Ceratitis capitata

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
31	-	-	Ceratitis capitata

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
20	-	between pH 6.0 and 9.0, stable for 1 h	Ceratitis capitata
30	-	30 min stable, pH 6.6	Ceratitis capitata
45	-	pH 6.6, 5 min stable	Ceratitis capitata
55	-	inactivation after 30 min	Ceratitis capitata
60	-	inactivation after 5 min	Ceratitis capitata

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
32	-	sn-glycerol 3-phosphate	per subunit of 33500 Da	Ceratitis capitata
188	-	dihydroxyacetone phosphate	per subunit of 33500 Da	Ceratitis capitata

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.6	-	optimum pH for NADH oxidation around pH 6.6	Ceratitis capitata
10	-	optimum pH for NAD+ reduction about pH 10.0	Ceratitis capitata

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.2	7.8	about half-maximal activity between pH 5.2 and 7.8, reduction of dihydroxyacetone phosphate	Ceratitis capitata
8	11	about half-maximal activity at pH 8.0 and 11.0, oxidation of L-glycerol-3-phosphate	Ceratitis capitata

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	-	inactivation after 1 h at 20°C	Ceratitis capitata
6	9	stable for 16 h at 20°C	Ceratitis capitata
10	-	inactivation after 1 h at 20°C	Ceratitis capitata

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Ceratitis capitata
NADH	-	Ceratitis capitata

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Release 2023.1 (January 2023)