Inhibitors | Comment | Organism | Structure |
---|---|---|---|
nitrate | 25 mM, competitive vs. glyoxylate | Neurospora crassa | |
p-chloromercuribenzoate | 1 mM, 70% inhibition | Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
NADPH | - |
Neurospora crassa | |
0.085 | - |
Hydroxypyruvate | - |
Neurospora crassa | |
0.23 | - |
NADH | - |
Neurospora crassa | |
5.7 | - |
glyoxylate | - |
Neurospora crassa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
gel filtration | Neurospora crassa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + NAD(P)H | Neurospora crassa | - |
glycolate + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
isoenzyme 2, protamine sulfate, DEAE-cellulose, hydroxylapatite, Sephadex G-150, DEAE-cellulose, phospho-cellulose | Neurospora crassa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
108.1 | - |
isoenzyme 2 | Neurospora crassa |
Storage Stability | Organism |
---|---|
4°C, alkaline pH, no loss of activity after 1 week, at pH 7-11, 20% loss of activity | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + NAD(P)H | - |
Neurospora crassa | glycolate + NAD(P)+ | - |
? | |
glyoxylate + NAD(P)H | isoenzyme 2, 16% activity with NADH | Neurospora crassa | glycolate + NAD(P)+ | - |
? | |
glyoxylate + NADH + H+ | - |
Neurospora crassa | glycolate + NAD+ | - |
r | |
hydroxypyruvate + NAD(P)H + H+ | 25 mM hydroxypyruvate, 68% of the activity with glyoxylate, 2.5 mM hydroxypyruvate, isoenzyme 2, 332% of the activity of glyoxylate | Neurospora crassa | glycerate + NAD(P)+ | - |
? | |
phenylpyruvate + NAD(P)H | isoenzyme 2, 6% activity of glyoxylate | Neurospora crassa | phenyllactate + NAD(P)+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
60% loss of activity after 10 min, activity is completely lost above 60°C | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
reduction of hydroxypyruvate | Neurospora crassa |
6.7 | - |
reduction of glyoxylate | Neurospora crassa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | NADPH is preferred | Neurospora crassa | |
NADPH | NADPH is preferred | Neurospora crassa |