Cloned (Comment) | Organism |
---|---|
gene mbdh, recombinant expression of the enzyme in Escherichia coli strain BL21(DE3) | Serratia sp. T241 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3S)-butane-2,3-diol + NAD+ | Serratia sp. T241 | - |
(S)-acetoin + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia sp. T241 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.14 | - |
recombinant enzyme, pH 8.0, 22°C | Serratia sp. T241 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3S)-butane-2,3-diol + NAD+ | - |
Serratia sp. T241 | (S)-acetoin + NADH + H+ | - |
? | |
additional information | meso-2,3-BDH from Serratia sp. T241 exhibits higher catalytic efficiency compared with the meso-2,3-BDHs from Klebsiella pneumoniae strain XJ-Li and Serratia marcescens strain H30. No activity is detected for (2R,3R)-2,3-BD as substrate by meso-2,3-BDH, but meso-2,3-BDH from Serratia sp. T241 can efficiently convert (2S,3S)-2,3-BD and meso-2,3-BD into (3S)-acetoin and (3R)-acetoin, respectively | Serratia sp. T241 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
(S,S)-2,3-BDH | - |
Serratia sp. T241 |
mbdh | - |
Serratia sp. T241 |
More | cf. EC 1.1.1.B20 | Serratia sp. T241 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Serratia sp. T241 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Serratia sp. T241 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Serratia sp. T241 |