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Literature summary for 1.1.1.71 extracted from

  • Brown, S.D.; Guss, A.M.; Karpinets, T.V.; Parks, J.M.; Smolin, N.; Yang, S.; Land, M.L.; Klingeman, D.M.; Bhandiwad, A.; Rodriguez, M. Jr.; Raman, B.; Shao, X.; Mielenz, J.R.; Smith, J.C.; Keller, M.; Lynd, L.R.
    Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum (2011), Proc. Natl. Acad. Sci. USA, 108, 13752-71375.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
P704L/H734R the ethanol tolerant phenotype of Clostridium thermocellum is primarily due to a mutated bifunctional acetaldehyde-CoA/alcohol dehydrogenase gene (adhE). The mutant displays a complete loss of NADH-dependent activity with concomitant acquisition of NADPH-dependent activity. The reduction in specific activity with respect to NADH is far greater (about 25fold less activity) than the increase with respect to NADPH. Although total ADH activity dropps by 25fold, ethanol production does not drop significantly between strains under these conditions Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus A0A0H3W5U9 bifunctional enzyme, C-terminal section belongs to the iron-containing alcohol dehydrogenase family, N-terminal section belongs to the aldehyde dehydrogenase family
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.025
-
wild-type, cofoactor NADPH, pH 7.6, 55°C Acetivibrio thermocellus
0.052
-
mutant P704L/H734R, cofoactor NADH, pH 7.6, 55°C Acetivibrio thermocellus
0.12
-
mutant P704L/H734R, cofoactor NADPH, pH 7.6, 55°C Acetivibrio thermocellus
2.7
-
wild-type, cofoactor NADH, pH 7.6, 55°C Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NADH + H+
-
Acetivibrio thermocellus ethanol + NAD+
-
?
acetaldehyde + NADPH + H+
-
Acetivibrio thermocellus ethanol + NADP+
-
?

Synonyms

Synonyms Comment Organism
AdhE
-
Acetivibrio thermocellus

Cofactor

Cofactor Comment Organism Structure
NADH
-
Acetivibrio thermocellus
NADPH the ethanol tolerant phenotype of Clostridium thermocellum is primarily due to a mutated bifunctional acetaldehyde-CoA/alcohol dehydrogenase gene (adhE). The mutant displays a complete loss of NADH-dependent activity with concomitant acquisition of NADPH-dependent activity Acetivibrio thermocellus