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Literature summary for 1.1.1.71 extracted from

  • Elleuche, S.; Fodor, K.; von der Heyde, A.; Klippel, B.; Wilmanns, M.; Antranikian, G.
    Group III alcohol dehydrogenase from Pectobacterium atrosepticum: insights into enzymatic activity and organization of the metal ion-containing region (2014), Appl. Microbiol. Biotechnol., 98, 4041-4051.
    View publication on PubMed
Search for more literature for 1.1.1.71

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15[pREP4] cells Pectobacterium atrosepticum

Protein Variants

Protein Variants Comment Organism
D194A the mutation results in substantial catalytic deficiency (2.8% compared to the wild type enzyme) Pectobacterium atrosepticum
H198A inactive Pectobacterium atrosepticum
H267A inactive Pectobacterium atrosepticum
H281A inactive Pectobacterium atrosepticum
H363A the mutant with full activity is not able to grow on butanal-containing medium Pectobacterium atrosepticum

Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition at 1 mM Pectobacterium atrosepticum
Fe2+ no activity at 1 mM Pectobacterium atrosepticum
Ni2+ 14% residual activity at 1 mM Pectobacterium atrosepticum
Zn2+ 14% residual activity at 1 mM Pectobacterium atrosepticum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ required Pectobacterium atrosepticum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42100
-
-
 Pectobacterium atrosepticum
43200
-
-
 Pectobacterium atrosepticum

Organism

Organism UniProt Comment Textmining
Pectobacterium atrosepticum U6CL97
-
-
Pectobacterium atrosepticum DSM18077 U6CL97
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 pg gel filtration Pectobacterium atrosepticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butanal + NADPH + H+ 44% activity compared to pentanal Pectobacterium atrosepticum 1-butanol + NAD(P)+
-
 ?
furfural + NADPH + H+ 5% activity compared to pentanal Pectobacterium atrosepticum (furan-2-yl)methanol + NAD(P)+
-
 ?
glycoaldehyde + NADPH + H+ 5% activity compared to pentanal Pectobacterium atrosepticum ?
-
 ?
heptanal + NADPH + H+ 56% activity compared to pentanal Pectobacterium atrosepticum 1-heptanol + NAD(P)+
-
 ?
hexanal + NADPH + H+ 62% activity compared to pentanal Pectobacterium atrosepticum 1-hexanol + NAD(P)+
-
 ?
methylglyoxal + NADPH + H+ 29% activity compared to pentanal Pectobacterium atrosepticum ?
-
 ?
additional information no activity with glyoxal, methanal, and ethanal Pectobacterium atrosepticum ?
-
 ?
additional information the enzyme shows no oxidative activities towards alcoholic compounds Pectobacterium atrosepticum ?
-
 ?
additional information no activity with glyoxal, methanal, and ethanal Pectobacterium atrosepticum DSM18077 ?
-
 ?
additional information the enzyme shows no oxidative activities towards alcoholic compounds Pectobacterium atrosepticum DSM18077 ?
-
 ?
additional information no activity with glyoxal, methanal, and ethanal Pectobacterium atrosepticum DSM 18077 ?
-
 ?
additional information the enzyme shows no oxidative activities towards alcoholic compounds Pectobacterium atrosepticum DSM 18077 ?
-
 ?
octanal + NADPH + H+ 55% activity compared to pentanal Pectobacterium atrosepticum 1-octanol + NAD(P)+
-
 ?
pentanal + NADPH + H+ 100% activity Pectobacterium atrosepticum 1-pentanol + NAD(P)+
-
 ?
propanal + NADPH + H+ 14% activity compared to pentanal Pectobacterium atrosepticum 1-propanol + NAD(P)+
-
 ?

Subunits

Subunits Comment Organism
? x * 42100, calculated from amino acid sequence Pectobacterium atrosepticum
? x * 43200, SDS-PAGE Pectobacterium atrosepticum

Synonyms

Synonyms Comment Organism
ADH
-
 Pectobacterium atrosepticum
NAD(P)+-dependent alcohol dehydrogenase
-
 Pectobacterium atrosepticum
YqhD
-
 Pectobacterium atrosepticum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pectobacterium atrosepticum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 50 30 to 60% of activity is observed between 20° and 30°C and 20% activity at 10 and 50°C, respectively Pectobacterium atrosepticum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 60 the enzyme is thermally stable when incubated for 30 min between 4 and 40°C. Incubation for 30 min at temperatures between 10 and 30 °C slightly increases the catalytic activity. Recombinant enzyme is completely inactivated at 50 and 60°C Pectobacterium atrosepticum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pectobacterium atrosepticum

pH Range

pH Minimum pH Maximum Comment Organism
6 8 the enzyme shows around 50% of activity at pH 6.0, pH 6.5, and pH 8.0 Pectobacterium atrosepticum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Pectobacterium atrosepticum

pI Value

Organism Comment pI Value Maximum pI Value
Pectobacterium atrosepticum calculated from amino acid sequence
-
 5.5