Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a fusion protein with (His)6-tag and 13 amino acid linker at the N-terminal | Thermococcus sibiricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NADP+ | 60°C, pH 10.5, cosubstrate: 2,5-hexandiol | Thermococcus sibiricus | |
0.02 | - |
NADPH | 60°C, pH 7.5, cosubstrate: pyruvaldehyde | Thermococcus sibiricus | |
1.5 | - |
2,3-Pentanedione | 60°C, pH 7.5 | Thermococcus sibiricus | |
13.1 | - |
(2S,5S)-2,5-hexanediol | 60°C, pH 10.5 | Thermococcus sibiricus | |
17.75 | - |
Pyruvaldehyde | 60°C, pH 7.5 | Thermococcus sibiricus | |
168 | - |
2-propanol | 60°C, pH 10.5 | Thermococcus sibiricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | activity is increased in the presence of NaCl and remains at the elevated level up to 4 M of NaCl. The rates of 2-propanol and 2,5-hexanediol oxidation are increased by more than 2fold after the addition of NaCl up to 1 M to the assay mixture and is retained at the increased level up to 4 M of NaCl | Thermococcus sibiricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus sibiricus | C6A190 | - |
- |
Thermococcus sibiricus DSM 12597 | C6A190 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,5S)-2,5-hexanediol + NADP+ | - |
Thermococcus sibiricus | ? + NADPH + H+ | - |
? | |
(2S,5S)-2,5-hexanediol + NADP+ | - |
Thermococcus sibiricus DSM 12597 | ? + NADPH + H+ | - |
? | |
2,3-pentanedione + NADPH + H+ | - |
Thermococcus sibiricus | ? + NADP+ | - |
? | |
2,3-pentanedione + NADPH + H+ | - |
Thermococcus sibiricus DSM 12597 | ? + NADP+ | - |
? | |
2-propanol + NADP+ | - |
Thermococcus sibiricus | acetone + NADPH + H+ | - |
? | |
2-propanol + NADP+ | - |
Thermococcus sibiricus DSM 12597 | acetone + NADPH + H+ | - |
? | |
pyruvaldehyde + NADPH + H+ | - |
Thermococcus sibiricus | lactaldehyde + NADP+ | - |
? | |
pyruvaldehyde + NADPH + H+ | - |
Thermococcus sibiricus DSM 12597 | lactaldehyde + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Thermococcus sibiricus |
Synonyms | Comment | Organism |
---|---|---|
TsAdh319 | - |
Thermococcus sibiricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Thermococcus sibiricus |
70 | - |
oxidation of (2S,5S)-2,5-hexanediol, in presence of 1 M NaCl | Thermococcus sibiricus |
83 | - |
oxidation of (2S,5S)-2,5-hexanediol, no addition of NaCl | Thermococcus sibiricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 75 | 50°C: about 50% of maximal activity, 75°C: about 60% of maximal activity, oxidation of (2S,5S)-2,5-hexanediol, in presence of 1 M NaCl | Thermococcus sibiricus |
65 | 86 | 65°C: about 50% of maximal activity, 86°C: about 50% of maximal activity, oxidation of (2S,5S)-2,5-hexanediol, no addition of NaCl | Thermococcus sibiricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal denaturation is fully irreversible and follows first-order kinetics | Thermococcus sibiricus |
80 | - |
activity begins to decrease after 2 h preincubation. The rate of 2-propanol oxidation is decreased 34% and 58% over 24 h incubation at 80°C in the presence or absence of 1 M NaCl, respectively. Calculated half-life values of the enzyme in buffer with or without 1 M NaCl are 37 vs. 20 h at 80°C, respectively | Thermococcus sibiricus |
90 | - |
activity begins to decrease after 30 min preincubation. The incubation at 90°C provokes 31% and 40% reduction of 2-propanol oxidation rate in the presence or absence of 1 M NaCl, respectively. Calculated half-life values of the enzyme in buffer with or without 1 M NaCl are 8 vs. 6 h at 90 °C, respectively | Thermococcus sibiricus |
102 | - |
Tm-value in presence of 1 M NaCl | Thermococcus sibiricus |
104 | - |
Tm-value in absence of NaCl | Thermococcus sibiricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
2-propanol | 60°C, pH 10.5 | Thermococcus sibiricus | |
1.4 | - |
2,3-Pentanedione | 60°C, pH 7.5 | Thermococcus sibiricus | |
1.51 | - |
NADPH | 60°C, pH 7.5, cosubstrate: pyruvaldehyde | Thermococcus sibiricus | |
2.02 | - |
NADP+ | 60°C, pH 10.5, cosubstrate: 2,5-hexandiol | Thermococcus sibiricus | |
2.04 | - |
Pyruvaldehyde | 60°C, pH 7.5 | Thermococcus sibiricus | |
2.2 | - |
(2S,5S)-2,5-hexanediol | 60°C, pH 10.5 | Thermococcus sibiricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Thermococcus sibiricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0032 | - |
2-propanol | 60°C, pH 10.5 | Thermococcus sibiricus | |
0.11 | - |
Pyruvaldehyde | 60°C, pH 7.5 | Thermococcus sibiricus | |
0.17 | - |
(2S,5S)-2,5-hexanediol | 60°C, pH 10.5 | Thermococcus sibiricus | |
1.33 | - |
2,3-Pentanedione | 60°C, pH 7.5 | Thermococcus sibiricus | |
76 | - |
NADPH | 60°C, pH 7.5, cosubstrate: pyruvaldehyde | Thermococcus sibiricus | |
101 | - |
NADP+ | 60°C, pH 10.5, cosubstrate: 2,5-hexandiol | Thermococcus sibiricus |