Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | stimulation | Gluconacetobacter liquefaciens |
General Stability | Organism |
---|---|
unstable to dialysis in a cold room, in presence of sulfhydryl compounds, EDTA, sucrose and/or glycerol | Gluconacetobacter liquefaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 1 mM, 29% inhibition | Gluconacetobacter liquefaciens | |
Hg2+ | 1 mM, 97% inhibition | Gluconacetobacter liquefaciens | |
NEM | 1 mM, 82% inhibition | Gluconacetobacter liquefaciens | |
PCMB | 1 mM, 94% inhibition | Gluconacetobacter liquefaciens | |
Sn2+ | 1 mM, 20% inhibition | Gluconacetobacter liquefaciens | |
sodium lauryl sulfate | 1 mM, 76% inhibition | Gluconacetobacter liquefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
NADP+ | - |
Gluconacetobacter liquefaciens | |
0.9 | - |
5-Dehydro-D-gluconate | - |
Gluconacetobacter liquefaciens | |
18 | - |
D-gluconate | - |
Gluconacetobacter liquefaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 1 mM, slight activation | Gluconacetobacter liquefaciens | |
Fe3+ | 1 mM, activation | Gluconacetobacter liquefaciens | |
Li+ | 1 mM, slight activation | Gluconacetobacter liquefaciens | |
Mg2+ | 1 mM, slight activation | Gluconacetobacter liquefaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
gel filtration | Gluconacetobacter liquefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconacetobacter liquefaciens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Gluconacetobacter liquefaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Gluconacetobacter liquefaciens |
Storage Stability | Organism |
---|---|
5°C, pH 7.5, 0.01 M potassium phosphate buffer, 3 d, complete loss of activity | Gluconacetobacter liquefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-dehydro-D-fructose + NADPH | 23% of the activity with 5-dehydro-D-gluconate | Gluconacetobacter liquefaciens | D-fructose + NADP+ | - |
? | |
5-dehydro-D-gluconate + NADPH | - |
Gluconacetobacter liquefaciens | D-gluconate + NADP+ | - |
r | |
D-fructose + NADPH | 42% of the activity with 5-dehydro-D-gluconate | Gluconacetobacter liquefaciens | 5-dehydro-D-fructose + NADP+ | - |
? | |
D-gluconate + NADP+ | - |
Gluconacetobacter liquefaciens | 5-dehydro-D-gluconate + NADPH | - |
r | |
D-glucono-delta-lactone + NADP+ | 14% of the activity with D-gluconate | Gluconacetobacter liquefaciens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
reduction of 5-dehydro-D-gluconate and oxidation of D-gluconate | Gluconacetobacter liquefaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 7.5, 10 min, stable up to | Gluconacetobacter liquefaciens |
70 | - |
10 min, pH 7.5, about 75% loss of activity | Gluconacetobacter liquefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
reduction of 5-dehydro-D-gluconate | Gluconacetobacter liquefaciens |
10 | - |
oxidation of D-gluconate in presence of NADP+ | Gluconacetobacter liquefaciens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8.5 | pH 4.5: about 70% of maximal activity, pH 8.5: about 60% of maximal activity, reduction of 5-dehydro-D-gluconate | Gluconacetobacter liquefaciens |
8 | 12 | pH 8.0: about 30% of maximal activity, pH 12.0: 35% of maximal activity, oxidation of D-gluconate | Gluconacetobacter liquefaciens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | cofactor | Gluconacetobacter liquefaciens | |
NADPH | cofactor | Gluconacetobacter liquefaciens |