Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.69 extracted from

  • Ameyama, M.; Chiyonobu, T.; Adachi, O.
    Purification and properties of 5-ketogluconate reductase from Gluconobacter liquefaciens (1974), Agric. Biol. Chem., 38, 1377-1382.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
EDTA stimulation Gluconacetobacter liquefaciens

General Stability

General Stability Organism
unstable to dialysis in a cold room, in presence of sulfhydryl compounds, EDTA, sucrose and/or glycerol Gluconacetobacter liquefaciens

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 1 mM, 29% inhibition Gluconacetobacter liquefaciens
Hg2+ 1 mM, 97% inhibition Gluconacetobacter liquefaciens
NEM 1 mM, 82% inhibition Gluconacetobacter liquefaciens
PCMB 1 mM, 94% inhibition Gluconacetobacter liquefaciens
Sn2+ 1 mM, 20% inhibition Gluconacetobacter liquefaciens
sodium lauryl sulfate 1 mM, 76% inhibition Gluconacetobacter liquefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
NADP+
-
Gluconacetobacter liquefaciens
0.9
-
5-Dehydro-D-gluconate
-
Gluconacetobacter liquefaciens
18
-
D-gluconate
-
Gluconacetobacter liquefaciens

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ 1 mM, slight activation Gluconacetobacter liquefaciens
Fe3+ 1 mM, activation Gluconacetobacter liquefaciens
Li+ 1 mM, slight activation Gluconacetobacter liquefaciens
Mg2+ 1 mM, slight activation Gluconacetobacter liquefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
gel filtration Gluconacetobacter liquefaciens

Organism

Organism UniProt Comment Textmining
Gluconacetobacter liquefaciens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Gluconacetobacter liquefaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Gluconacetobacter liquefaciens

Storage Stability

Storage Stability Organism
5°C, pH 7.5, 0.01 M potassium phosphate buffer, 3 d, complete loss of activity Gluconacetobacter liquefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-dehydro-D-fructose + NADPH 23% of the activity with 5-dehydro-D-gluconate Gluconacetobacter liquefaciens D-fructose + NADP+
-
?
5-dehydro-D-gluconate + NADPH
-
Gluconacetobacter liquefaciens D-gluconate + NADP+
-
r
D-fructose + NADPH 42% of the activity with 5-dehydro-D-gluconate Gluconacetobacter liquefaciens 5-dehydro-D-fructose + NADP+
-
?
D-gluconate + NADP+
-
Gluconacetobacter liquefaciens 5-dehydro-D-gluconate + NADPH
-
r
D-glucono-delta-lactone + NADP+ 14% of the activity with D-gluconate Gluconacetobacter liquefaciens ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
reduction of 5-dehydro-D-gluconate and oxidation of D-gluconate Gluconacetobacter liquefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
pH 7.5, 10 min, stable up to Gluconacetobacter liquefaciens
70
-
10 min, pH 7.5, about 75% loss of activity Gluconacetobacter liquefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
reduction of 5-dehydro-D-gluconate Gluconacetobacter liquefaciens
10
-
oxidation of D-gluconate in presence of NADP+ Gluconacetobacter liquefaciens

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8.5 pH 4.5: about 70% of maximal activity, pH 8.5: about 60% of maximal activity, reduction of 5-dehydro-D-gluconate Gluconacetobacter liquefaciens
8 12 pH 8.0: about 30% of maximal activity, pH 12.0: 35% of maximal activity, oxidation of D-gluconate Gluconacetobacter liquefaciens

Cofactor

Cofactor Comment Organism Structure
NADP+ cofactor Gluconacetobacter liquefaciens
NADPH cofactor Gluconacetobacter liquefaciens