Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Thermotoga neapolitana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | about 20% residual activity at 1 mM | Thermotoga neapolitana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | - |
D-fructose | at pH 6.5 and 90°C | Thermotoga neapolitana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | about 300% activity at 1 mM | Thermotoga neapolitana | |
Ca2+ | about 220% activity at 1 mM | Thermotoga neapolitana | |
Co2+ | about 400% activity at 1 mM | Thermotoga neapolitana | |
Mg2+ | about 150% activity at 1 mM | Thermotoga neapolitana | |
Ni2+ | about 230% activity at 1 mM | Thermotoga neapolitana | |
Zn2+ | about 110% activity at 1 mM | Thermotoga neapolitana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
4 * 36000, SDS-PAGE | Thermotoga neapolitana |
135000 | - |
gel filtration | Thermotoga neapolitana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + NADH + H+ | Thermotoga neapolitana | - |
D-mannitol + NAD+ | - |
? | |
D-fructose + NADH + H+ | Thermotoga neapolitana DSM 4359 | - |
D-mannitol + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga neapolitana | - |
- |
- |
Thermotoga neapolitana DSM 4359 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Thermotoga neapolitana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + NADH + H+ | - |
Thermotoga neapolitana | D-mannitol + NAD+ | - |
? | |
D-fructose + NADH + H+ | - |
Thermotoga neapolitana DSM 4359 | D-mannitol + NAD+ | - |
? | |
additional information | the enzyme shows no activity with xylitol, inositol, sorbitol, rhamnose, mannose and xylose, and with NADPH and NADP+ as cofactors | Thermotoga neapolitana | ? | - |
? | |
additional information | the enzyme shows no activity with xylitol, inositol, sorbitol, rhamnose, mannose and xylose, and with NADPH and NADP+ as cofactors | Thermotoga neapolitana DSM 4359 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 36000, SDS-PAGE | Thermotoga neapolitana |
Synonyms | Comment | Organism |
---|---|---|
MtDH | - |
Thermotoga neapolitana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Thermotoga neapolitana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 95 | more than 60% of the maximal activity between 80 and 95°C | Thermotoga neapolitana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | 90 | the enzyme records 85%, 50% and 10% of its initial activity after 4 h of exposure to 75, 80, 85 and 90°C, respectively. The enzyme almost does not lose the activity after 1 h of incubation at 75°C and still has half of initial activity after incubated at 90°C for 1 h | Thermotoga neapolitana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
180 | - |
D-fructose | at pH 6.5 and 90°C | Thermotoga neapolitana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Thermotoga neapolitana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | more than 60% of the maximal activity in the pH range 6.09.0 | Thermotoga neapolitana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermotoga neapolitana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
D-fructose | at pH 6.5 and 90°C | Thermotoga neapolitana |