Application | Comment | Organism |
---|---|---|
industry | best strain for expression of MDH in both laboratory and industrial applications is Escherichia coli BL21 (DE3) plysS | Pseudomonas fluorescens |
Cloned (Comment) | Organism |
---|---|
subcloned into vector pDEST110 and overexpressed in different strains of Escherichia coli (BL21 (DE3) plysS, JM109, Origami(DE3) or M15) | Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, SDS-PAGE | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
DSM 50106 | - |
Purification (Comment) | Organism |
---|---|
by Ni2+-NTA affinity chromatography | Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
63 | - |
MDH expressed in Escherichia coli strain BL21 (DE3) plysS, with fructose as substrate | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arabitol + NAD(P)+ | - |
Pseudomonas fluorescens | ? + NAD(P)H | - |
? | |
D-fructose + NAD(P)+ | - |
Pseudomonas fluorescens | ? + NAD(P)H | - |
? | |
isomaltulose + NAD(P)+ | - |
Pseudomonas fluorescens | ? + NAD(P)H | - |
? | |
additional information | among the Escherichia coli strains, BL21 (DE3) plysS exhibits the maximum expression level of MDH (11mg/L) | Pseudomonas fluorescens | ? | - |
? | |
sorbitol + NAD(P)+ | - |
Pseudomonas fluorescens | ? + NAD(P)H | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, SDS-PAGE | Pseudomonas fluorescens |
Synonyms | Comment | Organism |
---|---|---|
MDH | - |
Pseudomonas fluorescens |
NADH-dependent mannitol dehydrogenase | - |
Pseudomonas fluorescens |