Crystallization (Comment) | Organism |
---|---|
- |
Levilactobacillus brevis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
NADH | - |
Levilactobacillus brevis | |
0.18 | 0.27 | NAD+ | - |
Levilactobacillus brevis | |
16.3 | 79.2 | D-fructose | - |
Levilactobacillus brevis | |
60 | - |
D-mannitol | - |
Levilactobacillus brevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol + NAD+ | Levilactobacillus brevis | - |
D-fructose + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Levilactobacillus brevis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Levilactobacillus brevis |
Storage Stability | Organism |
---|---|
-16°C, 0.02 M sodium acetate buffer pH 6.0, 1 mM 2-mercaptoethanol, several weeks | Levilactobacillus brevis |
3°C, crystalline enzyme in ammonium sulfate, 2 months | Levilactobacillus brevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol + NAD+ | - |
Levilactobacillus brevis | D-fructose + NADH + H+ | - |
? | |
D-mannitol + NAD+ | enzyme highly specific for D-mannitol and D-fructose | Levilactobacillus brevis | D-fructose + NADH + H+ | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.3 | - |
D-fructose reduction | Levilactobacillus brevis |
8.6 | - |
D-mannitol oxidation | Levilactobacillus brevis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | - |
not active below, D-mannitol oxidation | Levilactobacillus brevis |
8 | - |
not active above, D-fructose reduction | Levilactobacillus brevis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | - |
Levilactobacillus brevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Levilactobacillus brevis | |
NADH | - |
Levilactobacillus brevis |