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Literature summary for 1.1.1.64 extracted from

  • McKeever, B.M.; Hawkins, B.K.; Geissler, W.M.; Wu, L.; Sheridan, R.P.; Mosley, R.T.; Andersson, S.
    Amino acid substitution of arginine 80 in 17beta-hydroxysteroid dehydrogenase type 3 and its effect on NADPH cofactor binding and oxidation/reduction kinetics (2002), Biochim. Biophys. Acta, 1601, 29-37.
    View publication on PubMed
Search for more literature for 1.1.1.64

Protein Variants

Protein Variants Comment Organism
R80I mutant enzyme shows negligible conversion of androst-4-ene-3,17-dione to testosterone. The Km-value for androstenedione is 80fold higher than the KM-value for the wild-type enzyme Homo sapiens
R80K conversion of androst-4-ene-3,17-dione to testosterone is reduced as compared to wild-type enzyme. The Km-value for androstenedione is 3.5fold higher than the KM-value for the wild-type enzyme Homo sapiens
R80L conversion of androst-4-ene-3,17-dione to testosterone is strongly reduced as compared to wild-type enzyme. The Km-value for androstenedione is 21.6fold higher than the KM-value for the wild-type enzyme Homo sapiens
R80M mutant enzyme shows negligible conversion of androst-4-ene-3,17-dione to testosterone. The Km-value for androstenedione is 90.7fold higher than the KM-value for the wild-type enzyme Homo sapiens
R80Q mutant enzyme shows negligible conversion of androst-4-ene-3,17-dione to testosterone. The Km-value for androstenedione is 61.5fold higher than the KM-value for the wild-type enzyme Homo sapiens
R80Y mutant enzyme shows negligible conversion of androst-4-ene-3,17-dione to testosterone. The Km-value for androstenedione is 85.1fold higher than the KM-value for the wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the KM-values for R80 mutant enzymes are higher than the Km-value of the wild-type enzyme: for R80K 3.5fold, for R80L 21.6fold, for R80M 90.7fold, for R80Q 61.5fold, for R80Y 61.5fold, for R80Y 85.1fold, for R80I 80fold Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
androst-4-ene-3,17-dione + NADPH Homo sapiens the enzyme plays a central role in the development of the male phenotype. Mutations that inactivate the enzyme give rise to a rare form of male pseudohermaphroditism, referred to as 17beta-HSD-3 deficiency testosterone + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
androst-4-ene-3,17-dione + NADPH
-
 Homo sapiens testosterone + NADP+
-
 ?
androst-4-ene-3,17-dione + NADPH the enzyme plays a central role in the development of the male phenotype. Mutations that inactivate the enzyme give rise to a rare form of male pseudohermaphroditism, referred to as 17beta-HSD-3 deficiency Homo sapiens testosterone + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
17beta-HSD-3
-
 Homo sapiens
17beta-hydroxysteroid dehydrogenase type 3
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH no activity with NADH Homo sapiens