Any feedback?
Literature summary for 1.1.1.64 extracted from
- Testicular 17beta-hydroxysteroid dehydrogenase: its distribution, purification and properties (1975), J. Steroid Biochem., 6, 361-363.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0083 | - |
NADPH | - |
Sus scrofa |  |
| 0.04 | - |
androstenedione | - |
Sus scrofa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35500 | - |
- |
Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 17beta-estradiol + NADP+ | Rattus norvegicus | - |
estrone + NADPH | - |
r | |
| 17beta-estradiol + NADP+ | Sus scrofa | - |
estrone + NADPH | - |
r | |
| testosterone + NADP+ | Rattus norvegicus | - |
androst-4-ene-3,17-dione + NADPH + H+ | - |
r | |
| testosterone + NADP+ | Sus scrofa | - |
androst-4-ene-3,17-dione + NADPH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, Sephadex G-100, DEAE-cellulose, Bio-Gel P-100 | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| testis | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0033 | - |
enzyme activity in smooth-surfaced microsomes | Rattus norvegicus |
| 67.7 | - |
- |
Sus scrofa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 6 months, no loss of activity | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 17beta-estradiol + NADP+ | - |
Rattus norvegicus | estrone + NADPH | - |
r | |
| 17beta-estradiol + NADP+ | - |
Sus scrofa | estrone + NADPH | - |
r | |
| testosterone + NADP+ | - |
Rattus norvegicus | androst-4-ene-3,17-dione + NADPH + H+ | - |
r | |
| testosterone + NADP+ | - |
Sus scrofa | androst-4-ene-3,17-dione + NADPH + H+ | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
in phosphate buffer at pH 7.4 | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | at 37°C | Sus scrofa |
| 8.5 | - |
at 50°C | Sus scrofa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Rattus norvegicus | |
| NADP+ | - |
Sus scrofa | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
