General Stability | Organism |
---|---|
very labile enzyme, loss of activity after storage for 2 weeks at -50°C | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
NADPH | - |
Sus scrofa | |
0.177 | - |
NADH | - |
Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Sus scrofa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
disc gel electrophoresis | Sus scrofa |
35400 | - |
1 * 35400, sucrose density gradient centrifugation | Sus scrofa |
35500 | - |
gel filtration | Sus scrofa |
36500 | - |
SDS-PAGE | Sus scrofa |
180000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
17beta-estradiol + NADP+ | Rattus norvegicus | - |
estrone + NADPH | - |
r | |
17beta-estradiol + NADP+ | Sus scrofa | - |
estrone + NADPH | - |
r | |
17beta-estradiol + NADP+ | Oryctolagus cuniculus | - |
estrone + NADPH | - |
r | |
17beta-estradiol + NADP+ | Ovis aries | - |
estrone + NADPH | - |
r | |
testosterone + NAD+ | Sus scrofa | NADP+ is preferred | androst-4-ene-3,17-dione + NADH + H+ | - |
r | |
testosterone + NADP+ | Rattus norvegicus | - |
androst-4-ene-3,17-dione + NADPH + H+ | - |
r | |
testosterone + NADP+ | Sus scrofa | - |
androst-4-ene-3,17-dione + NADPH + H+ | - |
r | |
testosterone + NADP+ | Oryctolagus cuniculus | - |
androst-4-ene-3,17-dione + NADPH + H+ | - |
r | |
testosterone + NADP+ | Ovis aries | - |
androst-4-ene-3,17-dione + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Ovis aries | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography on estrone-aminocaproate-Sepharose | Ovis aries |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
testosterone + NADP+ = androstenedione + NADPH + H+ | 4-pro-S-hydrogen atom of NADPH is transferred to the alpha-face of the steroid molecule | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
ovary | - |
Ovis aries | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17beta-estradiol + NADP+ | - |
Rattus norvegicus | estrone + NADPH | - |
r | |
17beta-estradiol + NADP+ | - |
Sus scrofa | estrone + NADPH | - |
r | |
17beta-estradiol + NADP+ | - |
Oryctolagus cuniculus | estrone + NADPH | - |
r | |
17beta-estradiol + NADP+ | - |
Ovis aries | estrone + NADPH | - |
r | |
testosterone + NAD+ | NADP+ is preferred | Sus scrofa | androst-4-ene-3,17-dione + NADH + H+ | - |
r | |
testosterone + NADP+ | - |
Rattus norvegicus | androst-4-ene-3,17-dione + NADPH + H+ | - |
r | |
testosterone + NADP+ | - |
Sus scrofa | androst-4-ene-3,17-dione + NADPH + H+ | - |
r | |
testosterone + NADP+ | - |
Oryctolagus cuniculus | androst-4-ene-3,17-dione + NADPH + H+ | - |
r | |
testosterone + NADP+ | - |
Ovis aries | androst-4-ene-3,17-dione + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35400, sucrose density gradient centrifugation | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Rattus norvegicus | |
NADP+ | - |
Sus scrofa | |
NADP+ | - |
Oryctolagus cuniculus | |
NADP+ | - |
Ovis aries |