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Literature summary for 1.1.1.64 extracted from

  • Inano, H.; Tamaoki, B.
    Testicular 17beta-hydroxysteroid dehydrogenas: molecular properties and reaction mechanism (1986), Steroids, 48, 1-26.
    View publication on PubMed

General Stability

General Stability Organism
very labile enzyme, loss of activity after storage for 2 weeks at -50°C Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
NADPH
-
Sus scrofa
0.177
-
NADH
-
Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Sus scrofa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
disc gel electrophoresis Sus scrofa
35400
-
1 * 35400, sucrose density gradient centrifugation Sus scrofa
35500
-
gel filtration Sus scrofa
36500
-
SDS-PAGE Sus scrofa
180000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
17beta-estradiol + NADP+ Rattus norvegicus
-
estrone + NADPH
-
r
17beta-estradiol + NADP+ Sus scrofa
-
estrone + NADPH
-
r
17beta-estradiol + NADP+ Oryctolagus cuniculus
-
estrone + NADPH
-
r
17beta-estradiol + NADP+ Ovis aries
-
estrone + NADPH
-
r
testosterone + NAD+ Sus scrofa NADP+ is preferred androst-4-ene-3,17-dione + NADH + H+
-
r
testosterone + NADP+ Rattus norvegicus
-
androst-4-ene-3,17-dione + NADPH + H+
-
r
testosterone + NADP+ Sus scrofa
-
androst-4-ene-3,17-dione + NADPH + H+
-
r
testosterone + NADP+ Oryctolagus cuniculus
-
androst-4-ene-3,17-dione + NADPH + H+
-
r
testosterone + NADP+ Ovis aries
-
androst-4-ene-3,17-dione + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-
Ovis aries
-
-
-
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography on estrone-aminocaproate-Sepharose Ovis aries

Reaction

Reaction Comment Organism Reaction ID
testosterone + NADP+ = androstenedione + NADPH + H+ 4-pro-S-hydrogen atom of NADPH is transferred to the alpha-face of the steroid molecule Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Oryctolagus cuniculus
-
ovary
-
Ovis aries
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
17beta-estradiol + NADP+
-
Rattus norvegicus estrone + NADPH
-
r
17beta-estradiol + NADP+
-
Sus scrofa estrone + NADPH
-
r
17beta-estradiol + NADP+
-
Oryctolagus cuniculus estrone + NADPH
-
r
17beta-estradiol + NADP+
-
Ovis aries estrone + NADPH
-
r
testosterone + NAD+ NADP+ is preferred Sus scrofa androst-4-ene-3,17-dione + NADH + H+
-
r
testosterone + NADP+
-
Rattus norvegicus androst-4-ene-3,17-dione + NADPH + H+
-
r
testosterone + NADP+
-
Sus scrofa androst-4-ene-3,17-dione + NADPH + H+
-
r
testosterone + NADP+
-
Oryctolagus cuniculus androst-4-ene-3,17-dione + NADPH + H+
-
r
testosterone + NADP+
-
Ovis aries androst-4-ene-3,17-dione + NADPH + H+
-
r

Subunits

Subunits Comment Organism
monomer 1 * 35400, sucrose density gradient centrifugation Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Rattus norvegicus
NADP+
-
Sus scrofa
NADP+
-
Oryctolagus cuniculus
NADP+
-
Ovis aries