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Literature summary for 1.1.1.62 extracted from

  • Kristan, K.; Stojan, J.; Moeller, G.; Adamski, J.; Rizner, T.L.
    Coenzyme specificity in fungal 17beta-hydroxysteroid dehydrogenase (2005), Mol. Cell. Endocrinol., 241, 80-87.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain JM107 Curvularia lunata

Protein Variants

Protein Variants Comment Organism
A50R site-directed mutagenesis, the mutant shows increased rates of NADPH dissociation and thus enhanced substrate oxidation with NADP+ compared to the wild-type enzyme Curvularia lunata
N51R site-directed mutagenesis, the mutant shows increased rates of NADP+ dissociation and thus enhanced substrate reduction with NADPH compared to the wild-type enzyme Curvularia lunata
T202I site-directed mutagenesis, inactive mutant due to cofactor binding deficiency Curvularia lunata
T202V site-directed mutagenesis, inactive mutant due to cofactor binding deficiency Curvularia lunata
Y49D site-directed mutagenesis, the mutant shows altered cofactor specificity preferring NAD+, resembling to the the cD1d subfamily of the short-chain dehydrogenase/reductase superfamily Curvularia lunata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of wild-type and mutant enzymes, Theorell-Chance reaction mechanism Curvularia lunata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-estrene-3,17-dione + NADPH Curvularia lunata
-
4-estrene-17beta-ol-3-one + NADP+
-
r
estrone + NADPH Curvularia lunata
-
17beta-estradiol + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Curvularia lunata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain JM107 by glutathione affinity chromatography Curvularia lunata

Reaction

Reaction Comment Organism Reaction ID
17beta-estradiol + NAD(P)+ = estrone + NAD(P)H + H+ cofactor binding residues are Tyr49, Thr202, Ala50, and Asn51, Theorell-Chance reaction mechanism Curvularia lunata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-estrene-3,17-dione + NADPH
-
Curvularia lunata 4-estrene-17beta-ol-3-one + NADP+
-
r
estrone + NADPH
-
Curvularia lunata 17beta-estradiol + NADP+
-
r

Synonyms

Synonyms Comment Organism
17beta-HSD
-
Curvularia lunata
17beta-hydroxysteroid dehydrogenase
-
Curvularia lunata
More the enzyme belongs to the cP1 subfamily of the short-chain dehydrogenase/reductase superfamily Curvularia lunata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Curvularia lunata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Curvularia lunata

Cofactor

Cofactor Comment Organism Structure
additional information cofactor binding mechanism and specificity involving residues Tyr49, Thr202, Ala50, and Asn51, overview Curvularia lunata
NAD+ mutant Y49D, not the wild-type enzyme Curvularia lunata
NADP+ dependent on Curvularia lunata
NADPH dependent on Curvularia lunata