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Literature summary for 1.1.1.61 extracted from

  • Yoshida, Y.; Sato, M.; Nagano, K.; Hasegawa, Y.; Okamoto, T.; Yoshimura, F.
    Production of 4-hydroxybutyrate from succinate semialdehyde in butyrate biosynthesis in Porphyromonas gingivalis (2015), Biochim. Biophys. Acta, 1850, 2582-2591.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Porphyromonas gingivalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
succinate semialdehyde with NADH as cosubstrate, at pH 8.0 and 37°C Porphyromonas gingivalis
0.41
-
NADH with NADH as cosubstrate, at pH 8.0 and 37°C Porphyromonas gingivalis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ most favorable metal ion for enzymatic activity Porphyromonas gingivalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
1 * 37000, SDS-PAGE Porphyromonas gingivalis
42000
-
gel filtration Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinate semialdehyde + NADH + H+ Porphyromonas gingivalis
-
4-hydroxybutanoate + NAD+
-
?
succinate semialdehyde + NADH + H+ Porphyromonas gingivalis ATCC 33277
-
4-hydroxybutanoate + NAD+
-
?
succinate semialdehyde + NADPH + H+ Porphyromonas gingivalis
-
4-hydroxybutanoate + NADP+
-
?
succinate semialdehyde + NADPH + H+ Porphyromonas gingivalis ATCC 33277
-
4-hydroxybutanoate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis
-
-
-
Porphyromonas gingivalis ATCC 33277
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography Porphyromonas gingivalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinate semialdehyde + NADH + H+
-
Porphyromonas gingivalis 4-hydroxybutanoate + NAD+
-
?
succinate semialdehyde + NADH + H+
-
Porphyromonas gingivalis ATCC 33277 4-hydroxybutanoate + NAD+
-
?
succinate semialdehyde + NADPH + H+
-
Porphyromonas gingivalis 4-hydroxybutanoate + NADP+
-
?
succinate semialdehyde + NADPH + H+
-
Porphyromonas gingivalis ATCC 33277 4-hydroxybutanoate + NADP+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 37000, SDS-PAGE Porphyromonas gingivalis

Synonyms

Synonyms Comment Organism
PGN 0724 protein
-
Porphyromonas gingivalis
succinate semialdehyde reductase
-
Porphyromonas gingivalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1329
-
succinate semialdehyde with NADH as cosubstrate, at pH 8.0 and 37°C Porphyromonas gingivalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Porphyromonas gingivalis

pH Range

pH Minimum pH Maximum Comment Organism
7 9 more than 80% activity between pH 7.0 and 9.0 Porphyromonas gingivalis

Cofactor

Cofactor Comment Organism Structure
NADH NADH is a more favorable cofactor for the enzyme than NADPH Porphyromonas gingivalis
NADPH NADH is a more favorable cofactor for the enzyme than NADPH Porphyromonas gingivalis