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Literature summary for 1.1.1.6 extracted from

  • Piattoni, C.; Figueroa, C.; Asencion Diez, M.; Parcerisa, I.; Antuna, S.; Comelli, R.; Guerrero, S.; Beccaria, A.; Iglesias, A.
    Production and characterization of Escherichia coli glycerol dehydrogenase as a tool for glycerol recycling (2013), Process Biochem., 48, 406-412.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene gldA from Escherichia coli strain JM109, expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3), subcloning in Escherichia coli TOP10 cells Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic study with different effectors, overview Escherichia coli
0.05
-
NADH pH 6.5, 30°C, recombinant enzyme Escherichia coli
0.07
-
NADH pH 6.5, 30°C, recombinant enzyme, in presence of 20 mM KCl Escherichia coli
0.08
-
NADH pH 6.5, 30°C, recombinant enzyme, in presence of 30 mM NH4Cl Escherichia coli
0.15
-
Glycerone pH 6.5, 30°C, recombinant enzyme Escherichia coli
0.22
-
Glycerone pH 6.5, 30°C, recombinant enzyme, in presence of 30 mM NH4Cl Escherichia coli
0.24
-
Glycerone pH 6.5, 30°C, recombinant enzyme, in presence of 20 mM KCl Escherichia coli
0.81
-
NAD+ pH 10.3, 30°C, recombinant enzyme Escherichia coli
1
-
NAD+ pH 10.3, 30°C, recombinant enzyme, in presence of 30 mM NH4Cl Escherichia coli
3.2
-
NAD+ pH 10.3, 30°C, recombinant enzyme, in presence of 20 mM KCl Escherichia coli
47
-
glycerol pH 10.3, 30°C, recombinant enzyme, in presence of 20 mM KCl Escherichia coli
76
-
glycerol pH 10.3, 30°C, recombinant enzyme Escherichia coli
81
-
glycerol pH 10.3, 30°C, recombinant enzyme, in presence of 30 mM NH4Cl Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates, less effective than NH4+ Escherichia coli
NH4+ activates, 4fold increase in Vmax produced by NH4Cl in the direction of glycerol oxidation, and 70fold reduction in the Km for dihydroxyaceton and 2fold increase in the Vmax with 30 mM NH4Cl added to the dihydroxyaceton reduction reaction Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39800
-
8 * 40000, recombinant His-tagged enzyme, SDS-PAGE, 8 * 39800, about, sequence calculation Escherichia coli
40000
-
8 * 40000, recombinant His-tagged enzyme, SDS-PAGE, 8 * 39800, about, sequence calculation Escherichia coli
320000
-
recombinant His-tagged enzyme, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol + NAD+ Escherichia coli
-
glycerone + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
gene gldA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by immobilized metal affinity chromatography or by heat shock treatment, both resulting in about the same protein purity of over 90% Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerol + NAD+
-
Escherichia coli glycerone + NADH + H+
-
r
additional information ability of the enzyme to use glycerol from biodiesel waste as substrate, overview Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
octamer 8 * 40000, recombinant His-tagged enzyme, SDS-PAGE, 8 * 39800, about, sequence calculation Escherichia coli

Synonyms

Synonyms Comment Organism
Gro dehydrogenase
-
Escherichia coli
GroDHase
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80 activity range, profile overview Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
dihydroxyaceton reduction reaction Escherichia coli
11
-
glycerol oxidation reaction Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
3 9 dihydroxyaceton reduction reaction, activity range, profile overview Escherichia coli
7.5 12 glycerol oxidation reaction, activity range, profile overview Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Escherichia coli
NADH
-
Escherichia coli

General Information

General Information Comment Organism
evolution the enzyme belongs to the medium-chain dehydrogenase/reductase, MDRase, superfamily Escherichia coli
metabolism in Escherichia coli, the enzyme catalyzes the first step in fermentative glycerol metabolism to produce dihydroxyacetone, biochemical transformation pathway of glycerol, overview Escherichia coli
physiological function GroDHase is mainly involved in Gro utilization as a carbon and energy source Escherichia coli