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Literature summary for 1.1.1.6 extracted from

  • Yamada-Onodera, K.; Yamamoto, H.; Emoto, E.; Kawahara, N.; Tani, Y.
    Characterisation of glycerol dehydrogenase from a methylotrophic yeast, Hansenula polymorpha Dl-1, and its gene cloning (2002), Acta Biotechnol., 22, 337-353.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2,2'dipyridyl 1 mM, complete inhibition Ogataea angusta
CuCl2 strong Ogataea angusta
FeCl3 strong Ogataea angusta
HgCl2 strong Ogataea angusta
p-hydroxymercuribenzoate 0.1 mM, strong Ogataea angusta
Phenanthroline 1 mM, complete inhibition Ogataea angusta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.87
-
dihydroxyacetone
-
Ogataea angusta
118
-
glycerol
-
Ogataea angusta

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
Ogataea angusta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
2 * 36000, SDS-PAGE Ogataea angusta
72000
-
gel filtration Ogataea angusta

Organism

Organism UniProt Comment Textmining
Ogataea angusta
-
induced by growth on methanol or glycerol
-
Ogataea angusta DL-1
-
induced by growth on methanol or glycerol
-

Purification (Commentary)

Purification (Comment) Organism
-
Ogataea angusta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3R)-2,3-butanediol + NAD+ better substrate than glycerol Ogataea angusta ? + NADH
-
?
(2R,3R)-2,3-butanediol + NAD+ better substrate than glycerol Ogataea angusta DL-1 ? + NADH
-
?
(R)-1,2-propanediol + NAD+ better substrate than glycerol Ogataea angusta ? + NADH
-
?
(R)-1,2-propanediol + NAD+ better substrate than glycerol Ogataea angusta DL-1 ? + NADH
-
?
glycerol + NAD+
-
Ogataea angusta glycerone + NADH + H+
-
r
glycerol + NAD+
-
Ogataea angusta DL-1 glycerone + NADH + H+
-
r
glycerone + NADH + H+
-
Ogataea angusta glycerol + NAD+
-
r
glycerone + NADH + H+
-
Ogataea angusta DL-1 glycerol + NAD+
-
r
additional information no substrate: 1,3-propanediol, ethanol, 1-propanol, 2-propanol, propionic acid, 1,4-butanediol, sorbitol, L-iditol. Stereospecificity for R-form Ogataea angusta ?
-
?
additional information no substrate: 1,3-propanediol, ethanol, 1-propanol, 2-propanol, propionic acid, 1,4-butanediol, sorbitol, L-iditol. Stereospecificity for R-form Ogataea angusta DL-1 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 36000, SDS-PAGE Ogataea angusta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Ogataea angusta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Ogataea angusta

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Ogataea angusta
NADH
-
Ogataea angusta