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Literature summary for 1.1.1.6 extracted from
- Purification and properties of glycerol:NAD+ 2-oxidoreductase (glycerol dehydrogenase) from Schizosaccharomyces pombe (1985), J. Gen. Microbiol., 131, 1581-1588.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | at 1 mM 140% of the activity without activator, at 10 mM 220% of the activity without activator | Schizosaccharomyces pombe |  |
| N-ethylmaleimide | at 0.1 mM 145% of the activity without activator | Schizosaccharomyces pombe | |
| NH4+ | - |
Schizosaccharomyces pombe | |
| p-chloromercuribenzoic acid | at 0.1 mM 133% of the activity without activator | Schizosaccharomyces pombe | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Schizosaccharomyces pombe | |
| dithiothreitol | - |
Schizosaccharomyces pombe | |
| EDTA | 10 mM | Schizosaccharomyces pombe | |
| L-cysteine | - |
Schizosaccharomyces pombe | |
| Li+ | competitive, forward reaction | Schizosaccharomyces pombe | |
| Na+ | competitive, forward reaction | Schizosaccharomyces pombe | |
| NADP+ | inhibits oxidation of glycerol | Schizosaccharomyces pombe | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
dihydroxyacetone | pH 6.0 | Schizosaccharomyces pombe | |
| 0.12 | - |
NADH | pH 6.0 | Schizosaccharomyces pombe | |
| 0.13 | - |
NAD+ | pH 10.0 | Schizosaccharomyces pombe | |
| 0.5 | - |
glycerol | pH 10.0 | Schizosaccharomyces pombe | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | soluble, isolation from crude cell extract | Schizosaccharomyces pombe | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | - |
Schizosaccharomyces pombe | |
| Li+ | - |
Schizosaccharomyces pombe | |
| Mn2+ | increases activity significantly | Schizosaccharomyces pombe | |
| Zn2+ | increases activity | Schizosaccharomyces pombe | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
8 * 47000, SDS-PAGE | Schizosaccharomyces pombe |
| 400000 | - |
gel filtration | Schizosaccharomyces pombe |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerol + NAD+ | Schizosaccharomyces pombe | - |
dihydroxyacetone + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizosaccharomyces pombe | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Schizosaccharomyces pombe |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.3 | - |
- |
Schizosaccharomyces pombe |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, long term storage | Schizosaccharomyces pombe |
| 4°C, in 2 M (NH4)2SO4, 1 M glycerol, several months | Schizosaccharomyces pombe |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2,3-butanetriol + NAD+ | - |
Schizosaccharomyces pombe | 1,3-dihydroxybutane-2-one + NADH | - |
? | |
| 1,2-butanediol + NAD+ | - |
Schizosaccharomyces pombe | 1-hydroxybutane-2-one + NADH | - |
r | |
| 1,2-propanediol + NAD+ | - |
Schizosaccharomyces pombe | hydroxyacetone + NADH | - |
r | |
| 2,3-butanediol + NAD+ | - |
Schizosaccharomyces pombe | 3-hydroxybutane-2-one + NADH | - |
r | |
| DL-glyceraldehyde + NAD+ | - |
Schizosaccharomyces pombe | 3-hydroxypyruvaldehyde + NADH | - |
? | |
| ethanediol + NAD+ | - |
Schizosaccharomyces pombe | glycolaldehyde + NADH | - |
? | |
| glycerol + NAD+ | - |
Schizosaccharomyces pombe | dihydroxyacetone + NADH + H+ | - |
? | |
| glycerol + NAD+ | - |
Schizosaccharomyces pombe | dihydroxyacetone + NADH + H+ | - |
r | |
| methylglyoxal + NADH | - |
Schizosaccharomyces pombe | lactaldehyde + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 47000, SDS-PAGE | Schizosaccharomyces pombe |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | reduction of dihydroxyacetone | Schizosaccharomyces pombe |
| 11 | 12 | oxidation of glycerol | Schizosaccharomyces pombe |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | pH 5.5: about 70% of maximal activity, pH 9.0: about 45% of maximal activity, reduction of dihydroxyacetone | Schizosaccharomyces pombe |
| 9 | 12 | pH 9.0: about 45% of maximal activity, pH 11-12: maximal activity, oxidation of glycerol | Schizosaccharomyces pombe |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Schizosaccharomyces pombe | |
| NADH | - |
Schizosaccharomyces pombe | |
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