Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells | Klebsiella aerogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 50.5% residual activity at 5 mM | Klebsiella aerogenes | |
EDTA | the enzyme activity is mostly lost (about 97%) after EDTA treatment | Klebsiella aerogenes | |
Fe3+ | 27.2% residual activity at 5 mM | Klebsiella aerogenes | |
Hg2+ | complete inhibition at 1 mM | Klebsiella aerogenes | |
K+ | 73.6% residual activity at 5 mM | Klebsiella aerogenes | |
Zn2+ | 61.9% residual activity at 5 mM | Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
NAD+ | at pH 11.0 and 25°C | Klebsiella aerogenes | |
10.3 | - |
ribitol | at pH 11.0 and 25°C | Klebsiella aerogenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 209% activity at 5 mM | Klebsiella aerogenes | |
Ca2+ | 245% activity at 5 mM | Klebsiella aerogenes | |
Mg2+ | 162% activity at 5 mM | Klebsiella aerogenes | |
additional information | not affected by Mn2+ and Co2+ | Klebsiella aerogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25800 | - |
- |
Klebsiella aerogenes |
110000 | - |
gel filtration | Klebsiella aerogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribitol + NAD+ | Klebsiella aerogenes | - |
D-ribulose + NADH + H+ | - |
? | |
ribitol + NAD+ | Klebsiella aerogenes KCTC 2190 | - |
D-ribulose + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | A0A0H3FYM1 | - |
- |
Klebsiella aerogenes KCTC 2190 | A0A0H3FYM1 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with NADP+ | Klebsiella aerogenes | ? | - |
? | |
additional information | no activity with NADP+ | Klebsiella aerogenes KCTC 2190 | ? | - |
? | |
ribitol + NAD+ | - |
Klebsiella aerogenes | D-ribulose + NADH + H+ | - |
? | |
ribitol + NAD+ | - |
Klebsiella aerogenes KCTC 2190 | D-ribulose + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 25800, calculated from amino acid sequence | Klebsiella aerogenes |
Synonyms | Comment | Organism |
---|---|---|
NAD+-dependent ribitol dehydrogenase | - |
Klebsiella aerogenes |
RDH | - |
Klebsiella aerogenes |
ribitol-specific dehydrogenase | - |
Klebsiella aerogenes |
ribitol: NAD+ 2-oxidoreductase | - |
Klebsiella aerogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Klebsiella aerogenes |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | the enzyme retains more than 90% of the maximum activity at 50°C, with more than 85% activity at 55°C, and more than 80% at 60°C | Klebsiella aerogenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | the enzyme shows half-lives of 25.7, 15.3, 7.5, 3.1, and 0.45 h at 40°C, 45°C, 50°C, 55°C, and 60°C, respectively | Klebsiella aerogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
11 | - |
- |
Klebsiella aerogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on | Klebsiella aerogenes |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30.9 | - |
ribitol | at pH 11.0 and 25°C | Klebsiella aerogenes |