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Literature summary for 1.1.1.56 extracted from

  • Fossitt, D.D.; Wood, W.A.
    Pentitol dehydrogenases of Aerobacter aerogenes (1966), Methods Enzymol., 9, 180-184.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
sulfhydryl compounds requirement Klebsiella aerogenes

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Klebsiella aerogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 0.02 M, requirement Klebsiella aerogenes

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50 150 for ribitol in crude extract Klebsiella aerogenes
50 200 for L-arabitol in crude extract Klebsiella aerogenes
270 280 for xylitol in crude extract Klebsiella aerogenes
23000
-
after crystallization Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arabitol + NAD+
-
Klebsiella aerogenes L-xylulose + NADH + H+
-
r
xylitol + NAD+
-
Klebsiella aerogenes D-xylulose + NADH + H+
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 sugar reduction Klebsiella aerogenes
8.5 9 alcohol oxidation Klebsiella aerogenes

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Klebsiella aerogenes
NADH
-
Klebsiella aerogenes