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Literature summary for 1.1.1.56 extracted from

  • Taylor, S.S.; Rigby, P.W.J.; Hartley, B.S.
    Ribitol dehydrogenase from Klebsiella aerogenes. Purification and subunit structure (1974), Biochem. J., 141, 693-700.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
NAD+ improves stability and reverses inhibition Klebsiella aerogenes
unstable in dilute solutions Klebsiella aerogenes

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol competitive to ribitol Klebsiella aerogenes
diethyldithiocarbamate 50% inhibition at a concentration of 50 mM Klebsiella aerogenes
dithiothreitol
-
Klebsiella aerogenes
Hg2+ protection by NAD+ Klebsiella aerogenes
o-phenanthroline 50% inhibition at a concentration of 50 mM Klebsiella aerogenes
p-chloromercuribenzoate
-
Klebsiella aerogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
NAD+ pH 11 Klebsiella aerogenes
5
-
ribitol pH 11 Klebsiella aerogenes
1000
-
xylitol pH 11 Klebsiella aerogenes

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for metal ions Klebsiella aerogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
4 * 27000, wild type enzyme, SDS-PAGE Klebsiella aerogenes
100000 110000 wild type, gel filtration Klebsiella aerogenes

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild type Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
83
-
wild type enzyme Klebsiella aerogenes

Storage Stability

Storage Stability Organism
-20°C wild type enzyme shows only slight loss of activity in phosphate buffer pH 7.0 Klebsiella aerogenes
2°C presence of NAD+ several days Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ribitol + NAD+
-
Klebsiella aerogenes D-ribulose + NADH
-
?
xylitol + NAD+
-
Klebsiella aerogenes D-xylulose + NADH + H+
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 27000, wild type enzyme, SDS-PAGE Klebsiella aerogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
-
Klebsiella aerogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11
-
alcohol oxidation Klebsiella aerogenes

pH Stability

pH Stability pH Stability Maximum Comment Organism
10 11 unstable at high pH values Klebsiella aerogenes

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Klebsiella aerogenes
NADH
-
Klebsiella aerogenes