General Stability | Organism |
---|---|
NAD+ improves stability and reverses inhibition | Klebsiella aerogenes |
unstable in dilute solutions | Klebsiella aerogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | competitive to ribitol | Klebsiella aerogenes | |
diethyldithiocarbamate | 50% inhibition at a concentration of 50 mM | Klebsiella aerogenes | |
dithiothreitol | - |
Klebsiella aerogenes | |
Hg2+ | protection by NAD+ | Klebsiella aerogenes | |
o-phenanthroline | 50% inhibition at a concentration of 50 mM | Klebsiella aerogenes | |
p-chloromercuribenzoate | - |
Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
NAD+ | pH 11 | Klebsiella aerogenes | |
5 | - |
ribitol | pH 11 | Klebsiella aerogenes | |
1000 | - |
xylitol | pH 11 | Klebsiella aerogenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for metal ions | Klebsiella aerogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
4 * 27000, wild type enzyme, SDS-PAGE | Klebsiella aerogenes |
100000 | 110000 | wild type, gel filtration | Klebsiella aerogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild type | Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
83 | - |
wild type enzyme | Klebsiella aerogenes |
Storage Stability | Organism |
---|---|
-20°C wild type enzyme shows only slight loss of activity in phosphate buffer pH 7.0 | Klebsiella aerogenes |
2°C presence of NAD+ several days | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribitol + NAD+ | - |
Klebsiella aerogenes | D-ribulose + NADH | - |
? | |
xylitol + NAD+ | - |
Klebsiella aerogenes | D-xylulose + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 27000, wild type enzyme, SDS-PAGE | Klebsiella aerogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
- |
Klebsiella aerogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
11 | - |
alcohol oxidation | Klebsiella aerogenes |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
10 | 11 | unstable at high pH values | Klebsiella aerogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Klebsiella aerogenes | |
NADH | - |
Klebsiella aerogenes |