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Literature summary for 1.1.1.54 extracted from

  • Malone, V.F.; Chastian, A.J.; Ohlsson, J.T.; Poneleit, L.S.; Nemecek-Marshall, M.; Fall, R.
    Characterization of a Pseudomonas putida allylic alcohol dehydrogenase induced by growth on 2-methyl-3-buten-2-ol (1999), Appl. Environ. Microbiol., 65, 2622-2630.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.54

Inhibitors

Inhibitors Comment Organism Structure
3-methyl-2-buten-1-al product inhibition, therefore the product must be removed via reaction with hydrazine during the enzyme assay Pseudomonas putida
dithiothreitol
-
 Pseudomonas putida
EDTA only at concentration of 100 mM 50% reduction Pseudomonas putida
N-ethylmaleimide
-
 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 benzyl alcohol
-
 Pseudomonas putida
0.007
-
 3-chloro-2-buten-1-ol
-
 Pseudomonas putida
0.011
-
 3-aminobenzyl alcohol
-
 Pseudomonas putida
0.012
-
 (R)-(+)-perillyl alcohol
-
 Pseudomonas putida
0.036
-
 cinnamyl alcohol
-
 Pseudomonas putida
0.04
-
 3-methyl-2-buten-1-ol
-
 Pseudomonas putida
0.044
-
 2-Buten-1-ol
-
 Pseudomonas putida
0.064
-
 NAD+ with 3-methyl-2-buten-1-ol Pseudomonas putida
0.118
-
 NAD+ with benzyl alcohol Pseudomonas putida
0.27
-
 3-methyl-1-butanol
-
 Pseudomonas putida
1.1
-
 1-butanol
-
 Pseudomonas putida
1.7
-
 3-methyl-3-buten-1-ol
-
 Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
additional information is not sensitive against metal ion chelators Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37700
-
 4 * 37700, SDS-PAGE Pseudomonas putida
151000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-methyl-2-buten-1-ol + NAD+ Pseudomonas putida can use substrate as sole carbon source 3-methyl-2-buten-1-al + NADH
-
 ?
3-methyl-2-buten-1-ol + NAD+ Pseudomonas putida MB-1 can use substrate as sole carbon source 3-methyl-2-buten-1-al + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida MB-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Reaction

Reaction Comment Organism Reaction ID
allyl alcohol + NADP+ = acrolein + NADPH + H+ 3-methyl-2-buten-1-ol-dependent inducible activity Pseudomonas putida
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.05
-
 purified enzyme, substrate 1-butanol Pseudomonas putida
0.08
-
 purified enzyme, substrate 3-methyl-3-buten-1-ol Pseudomonas putida
0.2
-
 purified enzyme, substrate 3-methyl-1-butanol Pseudomonas putida
3.7
-
 purified enzyme, substrate cinnamyl alcohol Pseudomonas putida
4.6
-
 purified enzyme, substrate 2-buten-1-ol Pseudomonas putida
7.7
-
 purified enzyme, substrate 3-methyl-2-buten-1-ol Pseudomonas putida
8.8
-
 purified enzyme, substrate (R)-(+)-perillyl alcohol Pseudomonas putida
16
-
 purified enzyme, substrate 3-chlor-2-buten-1-ol Pseudomonas putida
19.3
-
 purified enzyme, in glycine buffer pH 9.4 Pseudomonas putida
22
-
 purified enzyme, substrate benzyl alcohol Pseudomonas putida
38
-
 purified enzyme, substrate aromatic 3-aminobenzyl alcohol Pseudomonas putida
38.8
-
 purified enzyme, in bis-Tris-propane-hydrazine buffer pH 9.4 Pseudomonas putida

Storage Stability

Storage Stability Organism
-70°C, 50-65% ethylene glycol (v/v), frozen in liquid nitrogen, stable for several month Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-(+)-perillyl alcohol + NADP+
-
 Pseudomonas putida ?
-
 r
1-butanol + NADP+
-
 Pseudomonas putida ?
-
 r
2-buten-1-ol + NADP+
-
 Pseudomonas putida ?
-
 r
2-buten-2-ol + NADPH
-
 Pseudomonas putida but-2-enal
-
 ?
2-buten-2-ol + NADPH
-
 Pseudomonas putida MB-1 but-2-enal
-
 ?
3-aminobenzyl alcohol + NADP+
-
 Pseudomonas putida ?
-
 r
3-chloro-2-buten-1-ol + NADP+
-
 Pseudomonas putida 3-chloro-2-butenaldehyde + NADPH
-
 ?
3-chloro-2-buten-1-ol + NADP+
-
 Pseudomonas putida MB-1 3-chloro-2-butenaldehyde + NADPH
-
 ?
3-methyl-1-butanol + NADP+
-
 Pseudomonas putida ?
-
 r
3-methyl-2-buten-1-ol + NAD+
-
 Pseudomonas putida 3-methyl-2-buten-1-al + NADH
-
 ?
3-methyl-2-buten-1-ol + NAD+ can use substrate as sole carbon source Pseudomonas putida 3-methyl-2-buten-1-al + NADH
-
 ?
3-methyl-2-buten-1-ol + NAD+
-
 Pseudomonas putida MB-1 3-methyl-2-buten-1-al + NADH
-
 ?
3-methyl-2-buten-1-ol + NAD+ can use substrate as sole carbon source Pseudomonas putida MB-1 3-methyl-2-buten-1-al + NADH
-
 ?
3-methyl-3-buten-1-ol + NADP+
-
 Pseudomonas putida ?
-
 r
benzyl alcohol + NADP+
-
 Pseudomonas putida ?
-
 r
cinnamyl alcohol + NADP+
-
 Pseudomonas putida cinnamyl aldehyde + NADPH
-
 ?
cinnamyl alcohol + NADP+
-
 Pseudomonas putida MB-1 cinnamyl aldehyde + NADPH
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 37700, SDS-PAGE Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
 for reduction Pseudomonas putida
10
-
 for oxidation Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
9.4
-
 stability at pH 9.4, 25°C much higher than at reactions optimal pH of 10.0 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+ exhibits less than 5% of the activity with NADP+ Pseudomonas putida