Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.53 extracted from

  • Devendran, S.; Mendez-Garcia, C.; Ridlon, J.
    Identification and characterization of a 20beta-HSDH from the anaerobic gut bacterium Butyricicoccus desmolans ATCC 43058 (2017), J. Lipid Res., 58, 916-925 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.53

Cloned(Commentary)

Cloned (Comment) Organism
heterologously overexpressed in Escherichia coli Agathobaculum desmolans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
 cortisone pH and temperature not specified in the publication Agathobaculum desmolans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
134500
-
 gel filtration Agathobaculum desmolans

Organism

Organism UniProt Comment Textmining
Agathobaculum desmolans
-
-
-
Agathobaculum desmolans ATCC 43058
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Agathobaculum desmolans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11-deoxycortisol + NADH + H+ i.e. 4-pregnen-17,21-diol-3,20-dione, 49.8% of the activity compared to cortisone Agathobaculum desmolans ? + NAD+
-
 ?
11-deoxycortisol + NADH + H+ i.e. 4-pregnen-17,21-diol-3,20-dione, 49.8% of the activity compared to cortisone Agathobaculum desmolans ATCC 43058 ? + NAD+
-
 ?
20beta-hydroxycortisone + NADH + H+ under in vitro conditions, the enzyme catalyzes reductive reaction far more readily than oxidative reaction Agathobaculum desmolans cortisone + NADH + H+
-
 r
20beta-hydroxycortisone + NADH + H+ under in vitro conditions, the enzyme catalyzes reductive reaction far more readily than oxidative reaction Agathobaculum desmolans ATCC 43058 cortisone + NADH + H+
-
 r
corticosterone + NADH + H+ i.e. 4-pregnen-11beta,21-diol-3,20-dione, 39.8% of the activity compared to cortisone Agathobaculum desmolans ? + NAD+
-
 ?
corticosterone + NADH + H+ i.e. 4-pregnen-11beta,21-diol-3,20-dione, 39.8% of the activity compared to cortisone Agathobaculum desmolans ATCC 43058 ? + NAD+
-
 ?
cortisone + NADH + H+ the equilibrium for the recombinant enzyme clearly favors the reductive direction. Cortisol is the preferred substrate, as it is more rapidly converted to 20beta-dihydrocortisol relative to other structurally similar steroids tested Agathobaculum desmolans 20beta-hydroxycortisone + NAD+
-
 r
cortisone + NADH + H+ the equilibrium for the recombinant enzyme clearly favors the reductive direction. Cortisol is the preferred substrate, as it is more rapidly converted to 20beta-dihydrocortisol relative to other structurally similar steroids tested Agathobaculum desmolans ATCC 43058 20beta-hydroxycortisone + NAD+
-
 r
tetrahydrocortisol + NADH + H+ i.e. 5beta-pregnan-3alpha,11beta,17,21-tetrol-20-one, 49.2% of the activity compared to cortisone Agathobaculum desmolans ? + NAD+
-
 ?
tetrahydrocortisol + NADH + H+ i.e. 5beta-pregnan-3alpha,11beta,17,21-tetrol-20-one, 49.2% of the activity compared to cortisone Agathobaculum desmolans ATCC 43058 ? + NAD+
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 33800, SDS-PAGE Agathobaculum desmolans

Synonyms

Synonyms Comment Organism
20beta-HSDH
-
 Agathobaculum desmolans
desE
-
 Agathobaculum desmolans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 reductive direction Agathobaculum desmolans
8.5 9.25 oxidative direction Agathobaculum desmolans

pH Range

pH Minimum pH Maximum Comment Organism
4 10 steroid reduction is observed within the pH range 4.0-10, retaining 19.9% of maximal activity at pH 4.0 and 29.5% of maximal activity at pH 10.0 Agathobaculum desmolans

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Agathobaculum desmolans
NADH
-
 Agathobaculum desmolans