General Stability | Organism |
---|---|
unfolding through 0.4 M guanidine hydrochloride | Curvularia lunata |
unfolding through 1.2 M urea | Curvularia lunata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | addition of a neutral electrolyte, such as NaCl, shields repulsion interactions in the highly positively charged enzyme at pH 2.0, effects on the secondary structure by increasing NaCl concentrations at pH 2.0 and 25°C, overview | Curvularia lunata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Curvularia lunata | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-estrene-17beta-ol-3-one + NADP+ | - |
Curvularia lunata | 4-estrene-3,17-dione + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | secondary structure analysis at 25°C and different pH values | Curvularia lunata |
Synonyms | Comment | Organism |
---|---|---|
17 beta-hydroxysteroid dehydrogenase | - |
Curvularia lunata |
17beta-HSD | - |
Curvularia lunata |
More | the enzyme is a member of the short-chain dehydrogenase/reductase, SDR, superfamily | Curvularia lunata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Curvularia lunata |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
binding of the coenzyme NADPH to the enzyme causes local structural changes that do not significantly affect the thermal stability of this protein | Curvularia lunata |
60 | - |
enzyme oligomers are resistant to temperatures up to 60°C | Curvularia lunata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Curvularia lunata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Curvularia lunata |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | - |
25°C, 150300 mM NaCl, formation of soluble aggregates enriched in alpha-helical and beta-sheet structures, the soluble aggregates start to precipitate at higher temperatures and NaCl concentrations | Curvularia lunata |
7 | 9 | the native dimeric enzyme is thermally stable up to 40°C in this pH range | Curvularia lunata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Curvularia lunata | |
NADPH | binding of the coenzyme NADPH to the enzyme causes local structural changes that do not significantly affect the thermal stability of this protein | Curvularia lunata |