Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Dimethylsulfoxide | - |
Rattus norvegicus | |
dioxane | - |
Rattus norvegicus | |
ethanol | - |
Rattus norvegicus | |
glycerol | - |
Rattus norvegicus | |
Propyleneglycol | - |
Rattus norvegicus | |
Triton X-100 | - |
Rattus norvegicus | |
Tween-80 | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
NADPH | 5alpha-dihydrotestosterone as substrate, 3-beta-hydroxysteroid dehydrogenase activity | Rattus norvegicus | |
0.0021 | - |
NADPH | estrone as substrate, 17beta-hydroxysteroid dehydrogenase activity | Rattus norvegicus | |
0.0082 | - |
NADP+ | testosterone as substrate, 17beta-hydroxysteroid dehydrogenase activity | Rattus norvegicus | |
0.009 | - |
NADP+ | 3beta-hydroxy-5alpha-androstan-17-one as substrate, 3beta-hydroxysteroid dehydrogenase activity | Rattus norvegicus | |
0.14 | - |
5alpha-dihydrotestosterone | - |
Rattus norvegicus | |
0.31 | - |
testosterone | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
gel filtration | Rattus norvegicus |
Organic Solvent | Comment | Organism |
---|---|---|
dimethylsulfoxide | 3.3% v/v: inactivation 43% | Rattus norvegicus |
dioxane | 3.3% v/v: inactivation 90% | Rattus norvegicus |
Ethanol | 1.7% v/v: inactivation 22% | Rattus norvegicus |
Glycerol | 5% v/v: inactivation 26% | Rattus norvegicus |
propyleneglycol | 3.3% v/v: inactivation 50% | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Wistar | - |
Rattus norvegicus Wistar | - |
Wistar | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3beta-hydroxy-5alpha-androstan-17-one + NADP+ | - |
Rattus norvegicus | 5alpha-androstane-3,17-dione + NADPH | - |
? | |
5alpha-androstane-3beta,17beta-diol + NADP+ | - |
Rattus norvegicus | 3beta-hydroxy-5alpha-androstan-17-one + NADPH | - |
? | |
5alpha-dihydrotestosterone + NAD(P)H | - |
Rattus norvegicus | 5alpha-androstane-3beta,17beta-diol + NADP+ | - |
? | |
5alpha-dihydrotestosterone + NADP+ | - |
Rattus norvegicus | 5alpha-androstane-3,17-dione + NADPH + H+ | - |
? | |
5alpha-dihydrotestosterone + NADP+ | - |
Rattus norvegicus Wistar | 5alpha-androstane-3,17-dione + NADPH + H+ | - |
? | |
androst-5-ene-3beta,17beta-diol + NADP+ | - |
Rattus norvegicus | dehydroepiandrosterone + NADPH | - |
r | |
androst-5-ene-3beta,17beta-diol + NADP+ | - |
Rattus norvegicus Wistar | dehydroepiandrosterone + NADPH | - |
r | |
androsterone + NADPH + H+ | - |
Rattus norvegicus | 5alpha-androstane-3alpha,17beta-diol + NADP+ | - |
? | |
androsterone + NADPH + H+ | - |
Rattus norvegicus Wistar | 5alpha-androstane-3alpha,17beta-diol + NADP+ | - |
? | |
estradiol + NADP+ | - |
Rattus norvegicus | estrone + NADPH | - |
r | |
estradiol + NADP+ | - |
Rattus norvegicus Wistar | estrone + NADPH | - |
r | |
testosterone + NAD(P)+ | - |
Rattus norvegicus | androst-4-en-3,17-dione + NAD(P)H + H+ | - |
? | |
testosterone + NAD(P)+ | - |
Rattus norvegicus Wistar | androst-4-en-3,17-dione + NAD(P)H + H+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | reductase activity | Rattus norvegicus |
8.2 | - |
dehydrogenase activity | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
unstable under slightly alkaline conditions | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |