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Literature summary for 1.1.1.50 extracted from

  • Mundaca, R.A.; Moreno-Guzman, M.; Eguilaz, M.; Yanez-Sedeno, P.; Pingarron, J.M.
    Enzyme biosensor for androsterone based on 3alpha-hydroxysteroid dehydrogenase immobilized onto a carbon nanotubes/ionic liquid/NAD+ composite electrode (2012), Talanta, 99, 697-702.
    View publication on PubMed

Application

Application Comment Organism
analysis engineering of 3alpha-hydrosteroid biosensor for androsterone determination by immobilizing the enzyme 3alpha-hydroxysteroid dehydrogenase in a composite electrode platform constituted of a mixture of multi-walled carbon nanotubes, octylpyridiniumhexafluorophosphate ionic liquid and NAD+ cofactor.This configuration allows the fast, sensitive and stable electrochemical detection of the NADH generated in the enzyme reaction. Reaction is linear for androsterone in the 0.5–10 microM concentration range. The detection limit achieved is 0.15 microM Comamonas testosteroni

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni P80702
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Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
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Comamonas testosteroni
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Synonyms

Synonyms Comment Organism
3alpha-HSD
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Comamonas testosteroni
HSD28
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Comamonas testosteroni
hsdA
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Comamonas testosteroni