Literature summary for 1.1.1.50 extracted from

Rizner, T.L.; Lin, H.K.; Peehl, D.M.; Steckelbroeck, S.; Bauman, D.R.; Penning, T.M.
Human type 3 3alpha-hydroxysteroid dehydrogenase (aldo-keto reductase 1C2) and androgen metabolism in prostate cells (2003), Endocrinology, 144, 2922-2932.

Search for more literature for 1.1.1.50

Cloned(Commentary)

Cloned (Comment)	Organism
transient functional expression in COS-1 cells, stable functional expression in PC-3 cells and in LNCaP cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
NADPH	strong inhibition of 3alpha-androstanediol oxidation	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.022	-	(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide	pH 7.0, 37°C with NADP+	Homo sapiens
0.026	-	5alpha-dihydrotestosterone	pH 7.0, 37°C, with NAD(P)H	Homo sapiens
0.042	-	(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide	pH 7.0, 37°C with NAD+	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5alpha-dihydrotestosterone + NADH	Homo sapiens	reaction direction is regulated by the NAD+:NADPH relation in the cell	3alpha-androstanediol + NAD+	-	r

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	type 3 isozyme AKR1C2	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
prostate	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide + NAD+	-	Homo sapiens	?	-	r
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide + NADP+	-	Homo sapiens	?	-	r
3alpha-androstanediol + NAD+	oxidation utilizing NAD+ as a cofactor is the preferred reaction in vitro	Homo sapiens	5alpha-dihydrotestosterone + NADH	-	r
5alpha-dihydrotestosterone + NADH	reaction direction is regulated by the NAD+:NADPH relation in the cell	Homo sapiens	3alpha-androstanediol + NAD+	-	r

Synonyms

Synonyms	Comment	Organism
3alpha-hydroxysteroid dehydrogenase	-	Homo sapiens
AKR1C2	-	Homo sapiens
aldo-keto reductase 1C2	-	Homo sapiens
More	enzyme belongs to the AKR1C subfamily of the AKR superfamily of enzymes	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0038	-	5alpha-dihydrotestosterone	pH 7.0, 37°C, with NAD(P)H	Homo sapiens
0.004	-	(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide	pH 7.0, 37°C with NADP+	Homo sapiens
0.0167	-	(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide	pH 7.0, 37°C with NAD+	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	oxidation utilizing NAD+ as a cofactor is the preferred reaction in vitro	Homo sapiens
NADH	-	Homo sapiens
NADP+	-	Homo sapiens
NADPH	-	Homo sapiens

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Release 2023.1 (January 2023)