Cloned (Comment) | Organism |
---|---|
expression of type II isoform in Escherichia coli | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
androstanedione | NADPH, detection spectrophotometrically | Homo sapiens | |
0.006 | - |
5alpha-dihydrotestosterone | NADP+, detection radiometrically | Homo sapiens | |
0.012 | - |
testosterone | NADP+, detection radiometrically | Homo sapiens | |
0.017 | - |
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide | NADP+, detection spectrophotometrically | Homo sapiens | |
0.018 | - |
5alpha-dihydrotestosterone | NADPH, detection radiometrically | Homo sapiens | |
0.019 | - |
5alpha-dihydrotestosterone | NADPH, detection spectrophotometrically | Homo sapiens | |
0.019 | - |
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide | NADP+, detection radio- or fluorometrically | Homo sapiens | |
0.021 | - |
9,10-phenanthrenequinone | NADPH, detection spectrophotometrically | Homo sapiens | |
0.048 | - |
androsterone | NADP+, detection radiometrically | Homo sapiens | |
0.087 | - |
4-nitrobenzaldehyde | NADPH, detection spectrophotometrically | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | type 3 isoform in liver virtually identical with bile acid-binding protein | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
type 2 isoform | - |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | enzyme isoforms bind substrate in different ways | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
colon | - |
Homo sapiens | - |
heart | - |
Homo sapiens | - |
kidney | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
lung | - |
Homo sapiens | - |
additional information | type 2 isoform is expressed in several tissues | Homo sapiens | - |
ovary | - |
Homo sapiens | - |
prostate gland | - |
Homo sapiens | - |
skeletal muscle | - |
Homo sapiens | - |
small intestine | - |
Homo sapiens | - |
thymus | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0005 | - |
substrate androsterone with cofactor NADP+, detection radiometrically | Homo sapiens |
0.0013 | - |
substrate 5alpha-dihydrotestosterone with cofactor NADP+, detection radiometrically | Homo sapiens |
0.0025 | - |
substrate 5alpha-dihydrotestosterone with cofactor NADPH, detection spectrophotometrically | Homo sapiens |
0.0042 | - |
substrate 5alpha-dihydrotestosterone with cofactor NADPH, detection radiometrically | Homo sapiens |
0.0048 | - |
substrate 3alpha-androstanediol with cofactor NADP+, detection spectrophotometrically | Homo sapiens |
0.0085 | - |
substrate 3alpha-androstanediol with cofactor NADP+, detection radiometrically | Homo sapiens |
0.0111 | - |
substrate androstanedione with cofactor NADPH, detection spectrophotometrically | Homo sapiens |
0.0134 | - |
substrate 3alpha-androstanediol with cofactor NADP+, detection fluorometrically | Homo sapiens |
0.13 | - |
substrate 4-nitrobenzaldehyde with cofactor NADPH, detection spectrophotometrically | Homo sapiens |
0.538 | - |
substrate 9,10-phenanthrenequinone with cofactor NADPH, detection spectrophotometrically | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide + NADP+ | - |
Homo sapiens | ? | - |
? | |
5alpha-androstan-3alpha,17beta-diol + NAD(P)+ | - |
Homo sapiens | 17beta-hydroxy-5alpha-androstan-3-one + NADPH | - |
r | |
5alpha-androstan-3alpha,17beta-diol + NAD(P)H | - |
Homo sapiens | 5alpha-dihydrotestosterone + NAD(P)+ | - |
r | |
5alpha-dihydrotestosterone + NAD(P)H + H+ | - |
Homo sapiens | 5alpha-androstan-3alpha,17beta-diol + NAD(P)+ | - |
r | |
5alpha-dihydrotestosterone + NAD(P)H + H+ | - |
Homo sapiens | (3beta,5alpha,17beta)-androstane-3,17-diol + NAD(P)+ | - |
r | |
5beta-androstan-3,17-dione + NAD(P)H | - |
Homo sapiens | 5beta-androstan-17one-3-ol + NAD(P)+ | - |
r | |
5beta-androstan-3alpha,17beta-diol + NADP+ | - |
Homo sapiens | 17beta-hydroxy-5beta-androstan-3-one + NADPH | - |
r | |
9,10-phenanthrenequinone + NADPH | - |
Homo sapiens | ? | - |
? | |
androsterone + NAD(P)+ | - |
Homo sapiens | 5alpha-androstan-3,17-dione + NAD(P)H | - |
r | |
androsterone + NADP+ | - |
Homo sapiens | androstane-3,17-dione + NADPH | - |
? | |
additional information | can not catalyze conversion of 3alpha-androstanediol to 5alpha-dihydrotestosterone | Homo sapiens | ? | - |
? | |
additional information | type 3 isoform in liver virtually identical with bile acid-binding protein | Homo sapiens | ? | - |
? | |
p-nitrobenzaldehyde + NAD(P)H | - |
Homo sapiens | p-nitrobenzylalcohol + NAD(P)+ | - |
? | |
testosterone + NAD(P)+ | - |
Homo sapiens | androstenedione + NAD(P)H + H+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00783 | - |
androsterone | - |
Homo sapiens | |
0.0398 | - |
5alpha-dihydrotestosterone | detection fluorometrically | Homo sapiens | |
0.0757 | - |
testosterone | - |
Homo sapiens | |
0.0965 | - |
5alpha-dihydrotestosterone | detection spectrophotometrically | Homo sapiens | |
0.114 | - |
5alpha-dihydrotestosterone | detection radiometrically | Homo sapiens | |
0.203 | - |
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide | detection spectrophotometrically | Homo sapiens | |
0.32 | - |
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide | detection radiometrically | Homo sapiens | |
0.507 | - |
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide | detection fluorometrically | Homo sapiens | |
1.08 | - |
4-nitrobenzaldehyde | - |
Homo sapiens | |
1.9 | - |
androstanedione | detection spectrophotometrically | Homo sapiens | |
18.9 | - |
9,10-phenanthrenequinone | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |