General Stability | Organism |
---|---|
stable in phosphate 50 mM plus 10-20% glycerol at 4°C, while freezing and thawing results in a great loss of enzyme activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dexamethasone | - |
Homo sapiens | |
dexamethasone 21-acetate | - |
Homo sapiens | |
dexamethasone 21-hemisuccinate | - |
Homo sapiens | |
testosterone | at concentration 0.001 mM 75% inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
5beta-Dihydrocortisol | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-dihydrocortisol + NADPH | - |
Homo sapiens | 3alpha,5alpha-tetrahydrocortisol + NADP+ | - |
? | |
5alpha-dihydrotestosterone + NAD(P)H + H+ | - |
Homo sapiens | 5alpha-androstan-3alpha,17beta-diol + NAD(P)+ | - |
? | |
5alpha-dihydrotestosterone + NAD(P)H + H+ | - |
Homo sapiens | (3beta,5alpha,17beta)-androstane-3,17-diol + NAD(P)+ | - |
? | |
5beta-dihydrocortisol + NADPH | - |
Homo sapiens | 3alpha,5beta-tetrahydrocortisol + NADP+ | - |
? | |
5beta-dihydrotestosterone + NAD(P)H | - |
Homo sapiens | 5beta-androstan-3alpha,17beta-diol + NAD(P)+ | - |
? | |
additional information | a saturated 4,5 double bound is essential for enzyme activity, 3-ketosteroids are not used as substrates | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37000, SDS-PAGE | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.4 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | preference for | Homo sapiens |