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Literature summary for 1.1.1.50 extracted from
- NAD-dependent 3alpha- and 12alpha-hydroxysteroid dehydrogenase activities from Eubacterium lentum ATCC no. 25559 (1977), Biochim. Biophys. Acta, 489, 466-476.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | 0.0024 | 17beta-hydroxy-5alpha-androstan-3-one | also in this range: 3alpha,7alpha-dihydroxy-5beta-cholanoyl glycine | Eggerthella lenta |  |
| 0.007 | 0.0083 | 5alpha-dihydrotestosterone | also in this range: 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine | Eggerthella lenta | |
| 0.01 | 0.025 | cholic acid | NAD+, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine | Eggerthella lenta | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Eggerthella lenta | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 2 days | Eggerthella lenta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine + NAD+ | - |
Eggerthella lenta | 12alpha-hydroxy-3-oxo-5beta-cholanoyl glycine + NADH | - |
? | |
| 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine + NAD+ | - |
Eggerthella lenta | 7alpha,12alpha-dihydroxy-5beta-cholan-3-one-24-oyl glycine + NADH | - |
? | |
| 3alpha,7alpha-dihydroxy-5beta-cholanoyl glycine + NAD+ | - |
Eggerthella lenta | 7alpha-hydroxy-3-oxo-5beta-cholanoyl glycine + NADH | - |
? | |
| 3alpha-hydroxy-5beta-androstan-17-one + NADP+ | - |
Eggerthella lenta | 5beta-androstan-3,17-dione + NADPH | - |
r | |
| 5alpha-androstan-3alpha,17beta-diol + NAD(P)+ | - |
Eggerthella lenta | 17beta-hydroxy-5alpha-androstan-3-one + NADPH | - |
r | |
| 5beta-androstan-3alpha,17beta-diol + NADP+ | - |
Eggerthella lenta | 17beta-hydroxy-5beta-androstan-3-one + NADPH | - |
r | |
| androsterone + NAD(P)+ | i.e. 3alpha-hydroxy-5alpha-androstan-17-one | Eggerthella lenta | 5alpha-androstan-3,17-dione + NAD(P)H | - |
r | |
| cholic acid + NAD+ | - |
Eggerthella lenta | 3-oxocholic acid + NADH + H+ | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
30-60 min stable | Eggerthella lenta |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 11.4 | - |
- |
Eggerthella lenta |
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