Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the kcat value of the mutant enzymes is markedly reduced, resulting in a 10 and 18fold reduction in catalytic efficiency for NADP+ catalysis for mutant G6PDViangchan and mutant G6PDViangchan+Mahidol, respectively | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Trypsin | after 2 h of trypsin digestion in the presence of 0.01 mM NADP+ the enzyme retains about 57% of its original activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0072 | - |
NADP+ | at pH 8.0 and 37°C | Homo sapiens | |
0.0478 | - |
D-glucose 6-phosphate | at pH 8.0 and 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | Homo sapiens | - |
6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P11413 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap QXL column chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0443 | - |
crude extract, at pH 8.0 and 37°C | Homo sapiens |
0.2282 | - |
purified enzyme, at pH 8.0 and 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | - |
Homo sapiens | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G6PD | - |
Homo sapiens |
glucose-6-phosphate dehydrogenase | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | - |
the half-life temperature is 47°C | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
247 | - |
NADP+ | at pH 8.0 and 37°C | Homo sapiens | |
247 | - |
D-glucose 6-phosphate | at pH 8.0 and 37°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5200 | - |
D-glucose 6-phosphate | at pH 8.0 and 37°C | Homo sapiens | |
34300 | - |
NADP+ | at pH 8.0 and 37°C | Homo sapiens |