Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a target for anti-parasite drug development | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(4Z)-4-(3-bromobenzylidene)-1-(4-methylphenyl)pyrazolidine-3,5-dione | - |
Plasmodium falciparum | |
(5Z)-1-(4-bromophenyl)-5-[[5-(2-nitrophenyl)furan-2-yl]methylidene]pyrimidine-2,4,6(1H,3H,5H)-trione | - |
Plasmodium falciparum | |
(5Z)-1-(4-ethoxyphenyl)-5-[[5-(2-nitrophenyl)furan-2-yl]methylidene]pyrimidine-2,4,6(1H,3H,5H)-trione | - |
Plasmodium falciparum | |
(5Z)-5-[[5-(2-methyl-4-nitrophenyl)furan-2-yl]methylidene]-1-(3-methylphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione | reversible, non-competitive inhibition versus NADP+, mixed-type versus D-glucose 6-phosphate | Plasmodium falciparum | |
2-[(5Z)-5-(4-hydroxy-3-methoxybenzylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]-3-phenylpropanoate | - |
Plasmodium falciparum | |
2-[(E)-(4-nitrocyclohexa-2,4-dien-1-yl)(2-phenylhydrazinylidene)methyl]benzene-1,4-diol | non-competitive inhibition versus NADP+, non-competitive or mixed-type versus D-glucose 6-phosphate | Plasmodium falciparum | |
2-[4-(4-chlorophenyl)-1,3-thiazol-2-yl]-5-methyl-4-(3,4,5-trimethoxybenzyl)-2,4-dihydro-3H-pyrazol-3-one | irreversible, non-competitive inhibition versus NADP+, mixed-type versus D-glucose 6-phosphate | Plasmodium falciparum | |
3-(4-hydroxyphenyl)-2-(pyridin-3-yl)quinazolin-4(3H)-one | irreversible, non-competitive inhibition versus NADP+, mixed-type versus D-glucose 6-phosphate | Plasmodium falciparum | |
3-(5-bromopyridin-2-yl)-2-(pyridin-3-yl)quinazolin-4(3H)-one | irreversible, non-competitive inhibition versus NADP+, mixed-type versus D-glucose 6-phosphate | Plasmodium falciparum | |
3-(5-[(E)-[2-(5-nitropyridin-2-yl)hydrazinylidene]methyl]furan-2-yl)benzoic acid | - |
Plasmodium falciparum | |
4-fluoro-N-(4-hydroxynaphthalen-1-yl)benzenesulfonamide | - |
Plasmodium falciparum | |
4-methyl-3-(propan-2-yloxy)-6H-benzo[c]chromen-6-one | non-competitive inhibition versus NADP+, mixed-type versus D-glucose 6-phosphate | Plasmodium falciparum | |
5-[[5-(4-methoxy-2-nitrophenyl)furan-2-yl]methylidene]-2-thioxodihydropyrimidine-4,6(1H,5H)-dione | reversible, competitive inhibition versus NADP+, mixed-type versus D-glucose 6-phosphate | Plasmodium falciparum | |
additional information | high-throughput screening for small-molecule inhibitors of the enzyme from Plasmodium falciparum, inhibitor evaluation, structure-activity relationship analysis, overview | Plasmodium falciparum | |
N-(4-hydroxynaphthalen-1-yl)-2,5-dimethylbenzenesulfonamide | - |
Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0086 | - |
NADP+ | pH 7.5, temperature not specified in the publication | Plasmodium falciparum | |
0.0145 | - |
D-glucose 6-phosphate | pH 7.5, temperature not specified in the publication | Plasmodium falciparum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
107000 | - |
- |
Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | Plasmodium falciparum | - |
6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
additional information | Plasmodium falciparum | the enzyme is a bifunctional enzyme glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase, the enzyme activity is analyzed for the reaction of glucose-6-phosphate dehydrogenase, EC 1.1.1.49, in a coupled assay with diaphorase activity, which uses NADPH to reduce resazurin to form the highly fluorescent molecule resorufin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | - |
Plasmodium falciparum | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
additional information | the enzyme is a bifunctional enzyme glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase, the enzyme activity is analyzed for the reaction of glucose-6-phosphate dehydrogenase, EC 1.1.1.49, in a coupled assay with diaphorase activity, which uses NADPH to reduce resazurin to form the highly fluorescent molecule resorufin | Plasmodium falciparum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase | - |
Plasmodium falciparum |
PfGluPho | - |
Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Plasmodium falciparum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00097 | - |
pH 7.5, temperature not specified in the publication | Plasmodium falciparum | 4-methyl-3-(propan-2-yloxy)-6H-benzo[c]chromen-6-one | |
0.0018 | - |
pH 7.5, temperature not specified in the publication | Plasmodium falciparum | 2-[(E)-(4-nitrocyclohexa-2,4-dien-1-yl)(2-phenylhydrazinylidene)methyl]benzene-1,4-diol | |
0.0039 | - |
pH 7.5, temperature not specified in the publication | Plasmodium falciparum | 4-fluoro-N-(4-hydroxynaphthalen-1-yl)benzenesulfonamide | |
0.0053 | - |
pH 7.5, temperature not specified in the publication | Plasmodium falciparum | N-(4-hydroxynaphthalen-1-yl)-2,5-dimethylbenzenesulfonamide |
General Information | Comment | Organism |
---|---|---|
metabolism | the bifunctional glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase enzyme catalyzes the first and second steps in the parasite's pentose phosphate pathway, cf. EC 3.1.1.31 | Plasmodium falciparum |