Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.49 extracted from

  • Sentrk, M.; Ceyhun, S.B.; Erdogan, O.; Kfrevioglu, O.I.
    In vitro and in vivo effects of some pesticides on glucose-6-phosphate dehydrogenase enzyme activity from rainbow trout (Oncorhynchus mykiss) erythrocytes (2009), Pestic. Biochem. Physiol., 95, 95-99.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
cypermethrin inhibits G6PD in vitro Oncorhynchus mykiss
deltamethrin inhibits G6PD both in vivo and in vitro, significantly inhibits activity after the 48th hour. Among pesticides, it is the most effective one, which is widely used both at homes and in agricultural fields. Deltamethrin inhibits the enzyme at very low doses, particularly in in vivo conditions, indicating that fish in natural and cultural environments are susceptible to this pesticide and that deltamethrin contaminations can be cause high mortality in fish population, which may lead to the increase in food insufficiency for increasing populations and cause disruption of ecological balance. Thus, usage of deltamethrin must be well controlled Oncorhynchus mykiss
propoxur inhibits G6PD in vitro Oncorhynchus mykiss

Organism

Organism UniProt Comment Textmining
Oncorhynchus mykiss
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by ammonium sulfate precipitation and affinity chromatography, 1691fold, with a yield of 63% Oncorhynchus mykiss

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Oncorhynchus mykiss
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.24
-
purified enzyme Oncorhynchus mykiss

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate + NADP+
-
Oncorhynchus mykiss D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
G6PD
-
Oncorhynchus mykiss

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Oncorhynchus mykiss

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.84
-
deltamethrin
-
Oncorhynchus mykiss
2.63
-
cypermethrin
-
Oncorhynchus mykiss
16.55
-
propoxur
-
Oncorhynchus mykiss

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.63
-
-
Oncorhynchus mykiss cypermethrin
1.02
-
-
Oncorhynchus mykiss propoxur
12
-
-
Oncorhynchus mykiss deltamethrin