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Literature summary for 1.1.1.48 extracted from

  • Blachnitzky, E.O.; Wengenmayer, F.; Kurz, G.
    D-galactose dehydrogenase from Pseudomonas fluorescens. Purification, properties and structure (1974), Eur. J. Biochem., 47, 235-250.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
NAD+
-
Pseudomonas fluorescens
0.7
-
D-galactose
-
Pseudomonas fluorescens
2.3
-
NADP+
-
Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
alpha,alpha, 2 * 32000, SDS-PAGE Pseudomonas fluorescens
64000
-
gel filtration Pseudomonas fluorescens
102000
-
gel filtration Pelomonas saccharophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galactose + NAD+ Pseudomonas fluorescens
-
D-galactono-1,5-lactone + NADH
-
?
D-galactose + NAD+ Pelomonas saccharophila
-
D-galactono-1,5-lactone + NADH
-
?

Organism

Organism UniProt Comment Textmining
Pelomonas saccharophila
-
-
-
Pseudomonas fluorescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, CM-Sephadex, DEAE-Sephadex, hydroxyapatite, disc electrophoresis Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
849
-
-
Pseudomonas fluorescens

Storage Stability

Storage Stability Organism
-20°C, 100 mM phosphate buffer, pH 6.5-7.6, 30-50% glycerol, 18 months, no loss of activity Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-galactose + NAD+
-
Pseudomonas fluorescens 2-deoxy-galactono-1,5-lactone + NADH
-
?
2-deoxy-D-galactose + NAD+
-
Pelomonas saccharophila 2-deoxy-galactono-1,5-lactone + NADH
-
?
2-deoxy-D-galactose + NADP+
-
Pseudomonas fluorescens 2-deoxy-galactono-1,5-lactone + NADPH
-
?
6-deoxy-alpha-D-galactose + NAD+
-
Pseudomonas fluorescens 6-deoxy-galactono-1,5-lactone + NADH
-
?
6-deoxy-alpha-D-galactose + NAD+
-
Pelomonas saccharophila 6-deoxy-galactono-1,5-lactone + NADH
-
?
D-galactose + NAD+
-
Pseudomonas fluorescens D-galactono-1,5-lactone + NADH
-
?
D-galactose + NAD+
-
Pelomonas saccharophila D-galactono-1,5-lactone + NADH
-
?
L-arabinose + NAD+
-
Pseudomonas fluorescens L-arabinono-1,4-lactone + NADH
-
?
L-arabinose + NAD+
-
Pelomonas saccharophila L-arabinono-1,4-lactone + NADH
-
?

Subunits

Subunits Comment Organism
dimer alpha,alpha, 2 * 32000, SDS-PAGE Pseudomonas fluorescens
tetramer
-
Pelomonas saccharophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1180
-
D-galactose
-
Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.1 9.5
-
Pseudomonas fluorescens

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.6 9.2
-
Pseudomonas fluorescens