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Literature summary for 1.1.1.48 extracted from

  • Carlsson, H.; Ljung, S.; Bulow, L.
    Physical and kinetic effects on introduction of various linker regions in beta-galactosidase/galactose dehydrogenase fusion enzymes (1996), Biochim. Biophys. Acta, 1293, 154-160.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
beta-galactosidase-galactose dehydrogenase fusion enzymes, expressed in Escherichia coli Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
NAD+
-
Pseudomonas fluorescens
0.23
-
D-galactose
-
Pseudomonas fluorescens
0.25
-
NAD+ beta-galactosidase-galactose dehydrogenase fusion enzymes Pseudomonas fluorescens
0.37
-
D-galactose beta-galactosidase-galactose dehydrogenase fusion enzyme, 13 amino acids as linker Pseudomonas fluorescens
0.4
-
D-galactose beta-galactosidase-galactose dehydrogenase fusion enzyme, 9 amino acids as linker Pseudomonas fluorescens
0.45
-
D-galactose beta-galactosidase-galactose dehydrogenase fusion enzyme, 3 amino acids as linker Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galactose + NAD+ Pseudomonas fluorescens
-
D-galactono-1,5-lactone + NADH
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17
-
beta-galactosidase-galactose dehydrogenase fusion enzyme, 3 amino acids as linker Pseudomonas fluorescens
27
-
beta-galactosidase-galactose dehydrogenase fusion enzyme, 13 amino acids as linker Pseudomonas fluorescens
29
-
beta-galactosidase-galactose dehydrogenase fusion enzyme, 9 amino acids as linker Pseudomonas fluorescens
34
-
-
Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + NAD+
-
Pseudomonas fluorescens D-galactono-1,5-lactone + NADH
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
native enzyme, 70% activity after 20 min, 30% after 60 min, 20% after 90 min, beta-galactosidase-galactose dehydrogenase fusion enzymes, 80% activity after 90 min Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.9
-
-
Pseudomonas fluorescens