Cloned (Comment) | Organism |
---|---|
beta-galactosidase-galactose dehydrogenase fusion enzymes, expressed in Escherichia coli | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
NAD+ | - |
Pseudomonas fluorescens | |
0.23 | - |
D-galactose | - |
Pseudomonas fluorescens | |
0.25 | - |
NAD+ | beta-galactosidase-galactose dehydrogenase fusion enzymes | Pseudomonas fluorescens | |
0.37 | - |
D-galactose | beta-galactosidase-galactose dehydrogenase fusion enzyme, 13 amino acids as linker | Pseudomonas fluorescens | |
0.4 | - |
D-galactose | beta-galactosidase-galactose dehydrogenase fusion enzyme, 9 amino acids as linker | Pseudomonas fluorescens | |
0.45 | - |
D-galactose | beta-galactosidase-galactose dehydrogenase fusion enzyme, 3 amino acids as linker | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + NAD+ | Pseudomonas fluorescens | - |
D-galactono-1,5-lactone + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17 | - |
beta-galactosidase-galactose dehydrogenase fusion enzyme, 3 amino acids as linker | Pseudomonas fluorescens |
27 | - |
beta-galactosidase-galactose dehydrogenase fusion enzyme, 13 amino acids as linker | Pseudomonas fluorescens |
29 | - |
beta-galactosidase-galactose dehydrogenase fusion enzyme, 9 amino acids as linker | Pseudomonas fluorescens |
34 | - |
- |
Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + NAD+ | - |
Pseudomonas fluorescens | D-galactono-1,5-lactone + NADH | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
native enzyme, 70% activity after 20 min, 30% after 60 min, 20% after 90 min, beta-galactosidase-galactose dehydrogenase fusion enzymes, 80% activity after 90 min | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.9 | - |
- |
Pseudomonas fluorescens |