Application | Comment | Organism |
---|---|---|
biotechnology | the robust stability of the enzyme makes it an attractive participant for cofactor regeneration on practical applications, especially for the catalysis implemented in acidic pH and high temperature | Bacillus sp. (in: Bacteria) |
diagnostics | the enzyme can be a potential diagnostic reagent for blood glucose measurement | Bacillus sp. (in: Bacteria) |
Cloned (Comment) | Organism |
---|---|
heterogeneously expressed in Escherichia coli BL21 (DE3) | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.072 | - |
NAD+ | pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
0.404 | - |
NADP+ | pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
17.126 | - |
D-glucose | pH 8.0, 25°C | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | A0A068FPP9 | - |
- |
Bacillus sp. (in: Bacteria) ZJ | A0A068FPP9 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal affinity chromatography | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
194 | - |
pH 8.0, 25°C, D-glucose + NAD+ | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-galactose + NAD+ | 6.8% of the activity as compared to D-glucose | Bacillus sp. (in: Bacteria) | D-galactono-1,5-lactone + NADH | - |
? | |
beta-D-galactose + NAD+ | 6.8% of the activity as compared to D-glucose | Bacillus sp. (in: Bacteria) ZJ | D-galactono-1,5-lactone + NADH | - |
? | |
D-glucose + NAD+ | the enzyme prefers NAD+ rather than NADP+ | Bacillus sp. (in: Bacteria) | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose + NAD+ | the enzyme prefers NAD+ rather than NADP+ | Bacillus sp. (in: Bacteria) ZJ | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose + NADP+ | the enzyme prefers NAD+ rather than NADP+ | Bacillus sp. (in: Bacteria) | D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
D-glucose + NADP+ | the enzyme prefers NAD+ rather than NADP+ | Bacillus sp. (in: Bacteria) ZJ | D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
D-maltose + NAD+ | 10% of the activity as compared to D-glucose | Bacillus sp. (in: Bacteria) | ? | - |
? | |
D-maltose + NAD+ | 10% of the activity as compared to D-glucose | Bacillus sp. (in: Bacteria) ZJ | ? | - |
? | |
D-xylose + NADP+ | 6.1% of the activity as compared to D-glucose | Bacillus sp. (in: Bacteria) | D-xylono-1,5-lactone + NADPH + H+ | - |
? | |
D-xylose + NADP+ | 6.1% of the activity as compared to D-glucose | Bacillus sp. (in: Bacteria) ZJ | D-xylono-1,5-lactone + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 30000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
bzgdh | - |
Bacillus sp. (in: Bacteria) |
GDH | - |
Bacillus sp. (in: Bacteria) |
NAD(P)-dependent glucose 1-dehydrogenase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | 25°C: about 60% of maximal activity, 55°C: about 50% of maximal activity | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable at temperatures below 50°C | Bacillus sp. (in: Bacteria) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
73.96 | - |
NADP+ | pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
84.52 | - |
NAD+ | pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
87.84 | - |
D-glucose | pH 8.0, 25°C | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
glycine-NaOH buffer | Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | pH 7.0: about 40% of maximal activity, pH 10.0: about 45% of maximal activity | Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8.5 | 1 h incubation, the enzyme maintains around 80% of its initial activity | Bacillus sp. (in: Bacteria) |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme prefers NAD+ rather than NADP+ | Bacillus sp. (in: Bacteria) | |
NADP+ | the enzyme prefers NAD+ rather than NADP+ | Bacillus sp. (in: Bacteria) |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus sp. (in: Bacteria) | calculated from sequence | - |
5.4 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.13 | - |
D-glucose | pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
182.98 | - |
NADP+ | pH 8.0, 25°C | Bacillus sp. (in: Bacteria) | |
1166.29 | - |
NAD+ | pH 8.0, 25°C | Bacillus sp. (in: Bacteria) |