Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Lysinibacillus sphaericus |
Protein Variants | Comment | Organism |
---|---|---|
DS255 | the mutant displays significantly enhanced thermal stability with considerable soluble expression and high specific activity. It is extremely stable at pH ranging from 4.5 to 10.5, as it retains nearly 100% activity after incubating at different buffers for 1 h. The mutant also exhibits high thermostability, having a half-life of 9900 min at 50°C, which is 1868fold as that of the wild type enzyme | Lysinibacillus sphaericus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.072 | - |
NADP+ | wild type enzyme, at pH 8.0 and 25°C | Lysinibacillus sphaericus | |
0.094 | - |
NAD+ | wild type enzyme, at pH 8.0 and 25°C | Lysinibacillus sphaericus | |
0.197 | - |
NADP+ | mutant enzyme DS255, at pH 8.0 and 25°C | Lysinibacillus sphaericus | |
0.293 | - |
NAD+ | mutant enzyme DS255, at pH 8.0 and 25°C | Lysinibacillus sphaericus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
4 * 30000, SDS-PAGE | Lysinibacillus sphaericus |
120000 | - |
gel filtration | Lysinibacillus sphaericus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + NAD+ | Lysinibacillus sphaericus | - |
D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose + NAD+ | Lysinibacillus sphaericus G10 | - |
D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose + NADP+ | Lysinibacillus sphaericus | - |
D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
D-glucose + NADP+ | Lysinibacillus sphaericus G10 | - |
D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | C5IFU0 | - |
- |
Lysinibacillus sphaericus G10 | C5IFU0 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + NAD+ | - |
Lysinibacillus sphaericus | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose + NAD+ | - |
Lysinibacillus sphaericus G10 | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose + NADP+ | - |
Lysinibacillus sphaericus | D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
D-glucose + NADP+ | - |
Lysinibacillus sphaericus G10 | D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 30000, SDS-PAGE | Lysinibacillus sphaericus |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
Lysinibacillus sphaericus |
glucose 1-dehydrogenase | - |
Lysinibacillus sphaericus |
NAD(P)-dependent glucose 1-dehydrogenase | - |
Lysinibacillus sphaericus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the enzyme shows half-lives of 23100, 364.5, and 5.3 min after 10 min at 30°C, 40°C, and 50°C, respectively. The enzyme is inactive after 10 min at 60°C | Lysinibacillus sphaericus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
93 | - |
NADP+ | wild type enzyme, at pH 8.0 and 25°C | Lysinibacillus sphaericus | |
115 | - |
NAD+ | wild type enzyme, at pH 8.0 and 25°C | Lysinibacillus sphaericus | |
130 | - |
NADP+ | mutant enzyme DS255, at pH 8.0 and 25°C | Lysinibacillus sphaericus | |
180 | - |
NAD+ | mutant enzyme DS255, at pH 8.0 and 25°C | Lysinibacillus sphaericus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
in glycine-NaOH buffer | Lysinibacillus sphaericus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Lysinibacillus sphaericus | |
NADP+ | - |
Lysinibacillus sphaericus |