Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.47 extracted from

  • Gao, F.; Ding, H.; Xu, X.; Zhao, Y.
    A self-sufficient system for removal of synthetic dye by coupling of spore-displayed triphenylmethane reductase and glucose 1-dehydrogenase (2016), Environ. Sci. Pollut. Res. Int., 23, 21319-21326.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.344
-
NAD+ at pH 8.0 and 25°C Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + NAD+ Bacillus subtilis
-
D-glucono-1,5-lactone + NADH + H+
-
?
D-glucose + NADP+ Bacillus subtilis
-
D-glucono-1,5-lactone + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + NAD+
-
Bacillus subtilis D-glucono-1,5-lactone + NADH + H+
-
?
D-glucose + NADP+
-
Bacillus subtilis D-glucono-1,5-lactone + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
GDH
-
Bacillus subtilis
glucose 1-dehydrogenase
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 80 the enzyme shows considerable thermal stability after incubation for 30 min at temperature ranging from 25 to 80°C and maintains 20% of its maximum activity at 70°C Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
521000
-
NAD+ at pH 8.0 and 25°C Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10.5 the enzyme's activity is more than 60% of its maximum at pH values ranging from 5.5 to 10.5 Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10.5 the enzyme is extremely stable over a broad pH ranges from 4.0 to 10.5 and retains more than 80% of its original activity after incubation at pH values ranging from 4.5 to 10.5 Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bacillus subtilis
NADP+
-
Bacillus subtilis