Cloned (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
expression in Escherichia coli | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NAD+ | above 12 mM | Saccharolobus solfataricus | |
NADP+ | above 12 mM | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
beta-D-glucose | pH 6.5, 70°C, cosubstrate: NADP+ | Saccharolobus solfataricus | |
4.59 | - |
beta-D-glucose | pH 6.5, 70°C, cosubstrate: NAD+ | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + NAD(P)(+) | Saccharolobus solfataricus | the enzyme might represent the major player in glucose catabolism via the branched Entner-Doudoroff pathway | D-glucono-1,5-lactone + NAD(P)H | - |
ir | |
beta-D-glucose + NADP+ | Saccharolobus solfataricus | GDH-2 is absolutely specific for D-glucose. D-galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol were not used as substrates. kcat/Km of NADP+ is about 8fold higher compared to kcat/Km of NAD+ | D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97U21 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + NAD(P)(+) | the enzyme might represent the major player in glucose catabolism via the branched Entner-Doudoroff pathway | Saccharolobus solfataricus | D-glucono-1,5-lactone + NAD(P)H | - |
ir | |
beta-D-glucose + NAD+ | GDH-2 is absolutely specific for D-glucose. D-galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol were not used as substrates. kcat/Km for glucose in the reaction with NAD+ is about 8fold lower compared to kcat/Km of glucose in the reaction with NADP+ | Saccharolobus solfataricus | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
beta-D-glucose + NAD+ | the enzyme is absolutely specific for glucose. D-Galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol are not used as substrates. the catalytic efficiency for the reaction with beta-D-glucose and NADP+ (kcat/Km) is 7.8 fold higher compared to catalytic efficiency for the reaction with beta-D-glucose and NAD+ | Saccharolobus solfataricus | D-glucono-1,5-lactone + NADH | - |
ir | |
beta-D-glucose + NADP+ | GDH-2 is absolutely specific for D-glucose. D-galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol were not used as substrates. kcat/Km of NADP+ is about 8fold higher compared to kcat/Km of NAD+ | Saccharolobus solfataricus | D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
beta-D-glucose + NADP+ | GDH-2 is absolutely specific for D-glucose. D-galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol were not used as substrates. kcat/Km of glucose in the reaction with NADP+ is about 8fold higher compared to kcat/Km of glucose in reaction with NAD+ | Saccharolobus solfataricus | D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
beta-D-glucose + NADP+ | the enzyme is absolutely specific for glucose. D-Galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol are not used as substrates. the catalytic efficiency for the reaction with beta-D-glucose and NADP+ (kcat/Km) is 7.8 fold higher compared to catalytic efficiency for the reaction with beta-D-glucose and NAD+ | Saccharolobus solfataricus | D-glucono-1,5-lactone + NADPH | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
gdh-2 | - |
Saccharolobus solfataricus |
SSO3204 | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19.34 | - |
beta-D-glucose | pH 6.5, 70°C, cosubstrate: NADP+ | Saccharolobus solfataricus | |
68.23 | - |
beta-D-glucose | pH 6.5, 70°C, cosubstrate: NAD+ | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | catalytic efficiency for the reaction with beta-D-glucose and NADP+ (kcat/Km) is 7.8 fold higher compared to catalytic efficiency for the reaction with beta-D-glucose and NAD+ | Saccharolobus solfataricus | |
NAD+ | kcat/Km of glucose in reaction with NAD+ is about 8fold lower compared to kcat/Km of glucose in the reaction with NADP+ | Saccharolobus solfataricus | |
NADP+ | catalytic efficiency for the reaction with beta-D-glucose and NADP+ (kcat/Km) is 7.8 fold higher compared to catalytic efficiency for the reaction with beta-D-glucose and NAD+ | Saccharolobus solfataricus | |
NADP+ | kcat/Km of glucose in reaction with NADP+ is about 8fold higher compared to kcat/Km of glucose in the reaction with NAD+ | Saccharolobus solfataricus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in glucose catabolism via the branched EntnerDoudoroff pathway | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.86 | - |
beta-D-glucose | pH 6.5, 70°C, cosubstrate: NAD+ | Saccharolobus solfataricus | |
115.2 | - |
beta-D-glucose | pH 6.5, 70°C, cosubstrate: NADP+ | Saccharolobus solfataricus |