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Literature summary for 1.1.1.47 extracted from

  • Haferkamp, P.; Kutschki, S.; Treichel, J.; Hemeda, H.; Sewczyk, K.; Hoffmann, D.; Zaparty, M.; Siebers, B.
    An additional glucose dehydrogenase from Sulfolobus solfataricus: fine-tuning of sugar degradation? (2011), Biochem. Soc. Trans., 39, 77-81.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Saccharolobus solfataricus
expression in Escherichia coli Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
NAD+ above 12 mM Saccharolobus solfataricus
NADP+ above 12 mM Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
beta-D-glucose pH 6.5, 70°C, cosubstrate: NADP+ Saccharolobus solfataricus
4.59
-
beta-D-glucose pH 6.5, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-glucose + NAD(P)(+) Saccharolobus solfataricus the enzyme might represent the major player in glucose catabolism via the branched Entner-Doudoroff pathway D-glucono-1,5-lactone + NAD(P)H
-
ir
beta-D-glucose + NADP+ Saccharolobus solfataricus GDH-2 is absolutely specific for D-glucose. D-galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol were not used as substrates. kcat/Km of NADP+ is about 8fold higher compared to kcat/Km of NAD+ D-glucono-1,5-lactone + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97U21
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucose + NAD(P)(+) the enzyme might represent the major player in glucose catabolism via the branched Entner-Doudoroff pathway Saccharolobus solfataricus D-glucono-1,5-lactone + NAD(P)H
-
ir
beta-D-glucose + NAD+ GDH-2 is absolutely specific for D-glucose. D-galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol were not used as substrates. kcat/Km for glucose in the reaction with NAD+ is about 8fold lower compared to kcat/Km of glucose in the reaction with NADP+ Saccharolobus solfataricus D-glucono-1,5-lactone + NADH + H+
-
?
beta-D-glucose + NAD+ the enzyme is absolutely specific for glucose. D-Galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol are not used as substrates. the catalytic efficiency for the reaction with beta-D-glucose and NADP+ (kcat/Km) is 7.8 fold higher compared to catalytic efficiency for the reaction with beta-D-glucose and NAD+ Saccharolobus solfataricus D-glucono-1,5-lactone + NADH
-
ir
beta-D-glucose + NADP+ GDH-2 is absolutely specific for D-glucose. D-galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol were not used as substrates. kcat/Km of NADP+ is about 8fold higher compared to kcat/Km of NAD+ Saccharolobus solfataricus D-glucono-1,5-lactone + NADPH + H+
-
?
beta-D-glucose + NADP+ GDH-2 is absolutely specific for D-glucose. D-galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol were not used as substrates. kcat/Km of glucose in the reaction with NADP+ is about 8fold higher compared to kcat/Km of glucose in reaction with NAD+ Saccharolobus solfataricus D-glucono-1,5-lactone + NADPH + H+
-
?
beta-D-glucose + NADP+ the enzyme is absolutely specific for glucose. D-Galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol are not used as substrates. the catalytic efficiency for the reaction with beta-D-glucose and NADP+ (kcat/Km) is 7.8 fold higher compared to catalytic efficiency for the reaction with beta-D-glucose and NAD+ Saccharolobus solfataricus D-glucono-1,5-lactone + NADPH
-
ir

Synonyms

Synonyms Comment Organism
gdh-2
-
Saccharolobus solfataricus
SSO3204
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
19.34
-
beta-D-glucose pH 6.5, 70°C, cosubstrate: NADP+ Saccharolobus solfataricus
68.23
-
beta-D-glucose pH 6.5, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NAD+ catalytic efficiency for the reaction with beta-D-glucose and NADP+ (kcat/Km) is 7.8 fold higher compared to catalytic efficiency for the reaction with beta-D-glucose and NAD+ Saccharolobus solfataricus
NAD+ kcat/Km of glucose in reaction with NAD+ is about 8fold lower compared to kcat/Km of glucose in the reaction with NADP+ Saccharolobus solfataricus
NADP+ catalytic efficiency for the reaction with beta-D-glucose and NADP+ (kcat/Km) is 7.8 fold higher compared to catalytic efficiency for the reaction with beta-D-glucose and NAD+ Saccharolobus solfataricus
NADP+ kcat/Km of glucose in reaction with NADP+ is about 8fold higher compared to kcat/Km of glucose in the reaction with NAD+ Saccharolobus solfataricus

General Information

General Information Comment Organism
physiological function the enzyme is involved in glucose catabolism via the branched Entner–Doudoroff pathway Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
14.86
-
beta-D-glucose pH 6.5, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus
115.2
-
beta-D-glucose pH 6.5, 70°C, cosubstrate: NADP+ Saccharolobus solfataricus