Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10-100 mM, sodium phosphate buffer, pH 8.0 | Lysinibacillus sphaericus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Lysinibacillus sphaericus |
His-tagged version expressed in Escherichia coli BL21(DE3) | Lysinibacillus sphaericus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 60% inhibition at 50, 100 and 10 mM in sodium phosphate buffer at pH 8.0, Tris/HCl buffer at pH 8.0 and Tris/HCl buffer at pH 9.0, respectively | Lysinibacillus sphaericus | |
Cd2+ | more than 50% inhibition at 20 mM | Lysinibacillus sphaericus | |
Co2+ | more than 50% inhibition at 20 mM | Lysinibacillus sphaericus | |
Cu2+ | more than 50% inhibition at 20 mM | Lysinibacillus sphaericus | |
Fe2+ | complete inhibition at 10 mM | Lysinibacillus sphaericus | |
Fe3+ | complete inhibition at 10 mM | Lysinibacillus sphaericus | |
Mn2+ | more than 50% inhibition at 20 mM | Lysinibacillus sphaericus | |
Zn2+ | complete inhibition at 10 mM | Lysinibacillus sphaericus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.072 | - |
NADP+ | pH 8.0, 25°C | Lysinibacillus sphaericus | |
0.094 | - |
NAD+ | pH 8.0, 25°C | Lysinibacillus sphaericus | |
5.1 | - |
D-glucose | with NADP+, pH 8.0, 25°C | Lysinibacillus sphaericus | |
6 | - |
D-glucose | with NAD+, pH 8.0, 25°C | Lysinibacillus sphaericus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | inhibits enzymatic activity | Lysinibacillus sphaericus | |
Co2+ | inhibits enzymatic activity | Lysinibacillus sphaericus | |
Cu2+ | inhibits enzymatic activity | Lysinibacillus sphaericus | |
EDTA | enhances activity | Lysinibacillus sphaericus | |
Fe2+ | inhibits enzymatic activity | Lysinibacillus sphaericus | |
Fe3+ | inhibits enzymatic activity | Lysinibacillus sphaericus | |
K+ | enhances activity | Lysinibacillus sphaericus | |
K+ | 50-200 mM, Tris/HCl buffer pH 9.0 | Lysinibacillus sphaericus | |
Li+ | enhances activity | Lysinibacillus sphaericus | |
Li+ | 50-200 mM, Tris/HCl buffer pH 9.0 | Lysinibacillus sphaericus | |
Mg2+ | enhances activity | Lysinibacillus sphaericus | |
Mg2+ | 1-200 mM, Tris/HCl buffer pH 8.0 | Lysinibacillus sphaericus | |
Mn2+ | inhibits enzymatic activity | Lysinibacillus sphaericus | |
Na+ | enhances activity | Lysinibacillus sphaericus | |
Na+ | 50-200 mM, Tris/HCl buffer pH 9.0 | Lysinibacillus sphaericus | |
NH4+ | enhances activity | Lysinibacillus sphaericus | |
NH4+ | 50-200 mM, Tris/HCl buffer pH 9.0 | Lysinibacillus sphaericus | |
Zn2+ | inhibits enzymatic activity | Lysinibacillus sphaericus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27800 | - |
calculated from cDNA | Lysinibacillus sphaericus |
28000 | - |
SDS-PAGE | Lysinibacillus sphaericus |
120000 | - |
gel filtration | Lysinibacillus sphaericus |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 30%: nearly 100% activity after 1h at 25°C | Lysinibacillus sphaericus |
Acetone | enzyme exhibited high tolerance to 30% acetone | Lysinibacillus sphaericus |
DMSO | 60%: nearly 100% activity after 1h at 25°C | Lysinibacillus sphaericus |
DMSO | enzyme exhibited high tolerance to 60% DMSO | Lysinibacillus sphaericus |
Ethanol | 30%: nearly 100% activity after 1h at 25°C | Lysinibacillus sphaericus |
Ethanol | enzyme exhibited high tolerance to 30% ethanol | Lysinibacillus sphaericus |
isopropanol | 30%: nearly 100% activity after 1h at 25°C | Lysinibacillus sphaericus |
isopropanol | enzyme exhibits high tolerance to 30% isopropanol | Lysinibacillus sphaericus |
Methanol | 30%: nearly 100% activity after 1h at 25°C | Lysinibacillus sphaericus |
Methanol | enzyme exhibited high tolerance to 30% methanol | Lysinibacillus sphaericus |
n-hexane | 30%: nearly 100% activity after 1h at 25°C | Lysinibacillus sphaericus |
n-hexane | enzyme exhibits high tolerance to 30% n-hexane | Lysinibacillus sphaericus |
n-hexanol | 60%: nearly 100% activity after 1h at 25°C | Lysinibacillus sphaericus |
n-hexanol | enzyme exhibited high tolerance to 60% n-hexanol | Lysinibacillus sphaericus |
n-propanol | 10%: nearly 100% activity after 1h at 25°C | Lysinibacillus sphaericus |
n-propanol | enzyme exhibits high tolerance to 10% n-propanol | Lysinibacillus sphaericus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | C5IFU0 | - |
- |
Lysinibacillus sphaericus G10 | C5IFU0 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+) | Lysinibacillus sphaericus |
Storage Stability | Organism |
---|---|
-20°C, 25 mM sodium phosphate buffer (pH 6.5) with 20% glycerol (w/v), 1 year, no loss of activity | Lysinibacillus sphaericus |
30°C, 25 mM sodium phosphate buffer (pH 6.5), 1 week | Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + NAD+ | relative activity: 17.3% | Lysinibacillus sphaericus | D-galactono-1,5-lactone + NADH + H+ | - |
? | |
D-galactose + NAD+ | relative activity: 17.3% | Lysinibacillus sphaericus G10 | D-galactono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose + NAD+ | relative activity: 100% | Lysinibacillus sphaericus | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose + NAD+ | about 20% activity with D-galactose, D-xylose, maltose | Lysinibacillus sphaericus | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose + NAD+ | relative activity: 100% | Lysinibacillus sphaericus G10 | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose + NAD+ | about 20% activity with D-galactose, D-xylose, maltose | Lysinibacillus sphaericus G10 | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose + NADP+ | - |
Lysinibacillus sphaericus | D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
D-glucose + NADP+ | - |
Lysinibacillus sphaericus G10 | D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
D-lactose + NAD+ | relative activity: 4.2% | Lysinibacillus sphaericus | D-lactono-1,5-lactone + NADH + H+ | - |
? | |
D-maltose + NAD+ | relative activity: 22.4% | Lysinibacillus sphaericus | ? | - |
? | |
D-mannose + NAD+ | relative activity: 7.6% | Lysinibacillus sphaericus | D-mannono-1,5-lactone + NADH + H+ | - |
? | |
D-xylose + NAD+ | relative activity: 22.5% | Lysinibacillus sphaericus | D-xylono-1,5-lactone + NADH + H+ | - |
? | |
D-xylose + NAD+ | relative activity: 22.5% | Lysinibacillus sphaericus G10 | D-xylono-1,5-lactone + NADH + H+ | - |
? | |
sucrose + NAD+ | relative activity: 6.3% | Lysinibacillus sphaericus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 28000, SDS-PAGE, 120000 kDa by gel filtration, calculated: 261 amino acid residues, 27.8 kDa | Lysinibacillus sphaericus |
homotetramer | gel filtration, 4 * 28000 | Lysinibacillus sphaericus |
Synonyms | Comment | Organism |
---|---|---|
alkali-resistant glucose 1-dehydrogenase | - |
Lysinibacillus sphaericus |
GDH | - |
Lysinibacillus sphaericus |
glucose 1-dehydrogenase | - |
Lysinibacillus sphaericus |
LsGDH | - |
Lysinibacillus sphaericus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Lysinibacillus sphaericus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 35 | stable for 1 week at 30°C (pH 6.5), completely inactivated in 30 min at 55°C, limited protection by glycerol, 84% activity after 30 min at 65°C in the presence of 2 M NaCl, | Lysinibacillus sphaericus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
93 | - |
NAD+ | pH 8.0, 25°C | Lysinibacillus sphaericus | |
93 | - |
NADP+ | pH 8.0, 25°C | Lysinibacillus sphaericus | |
115 | - |
NAD+ | pH 8.0, 25°C | Lysinibacillus sphaericus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Lysinibacillus sphaericus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10.5 | - |
Lysinibacillus sphaericus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | - |
Lysinibacillus sphaericus |
6.5 | 10.5 | most stable at pH 8.5, retains more than 80% activity at pH 6.5-10.5 after 1 h at 25°C, more than 30% activity after 24 h at pH 10.0 | Lysinibacillus sphaericus |
10 | - |
at pH 10 for 24 h enzyme retains 30% of its activity | Lysinibacillus sphaericus |