Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Saccharolobus solfataricus |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour diffusion using a mother liquor of 12% (v/v) polyethylene glycol 4000, 0.1 M MES (pH 5.8), and 3% (v/v) propan-2-ol | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
T41A | reduced activity | Saccharolobus solfataricus |
T41V | reduced activity | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
D-xylose | wild type enzyme, NADP+ as cofactor | Saccharolobus solfataricus | |
0.25 | - |
D-xylose | wild type enzyme, NAD+ as cofactor | Saccharolobus solfataricus | |
0.44 | - |
beta-D-galactose | wild type enzyme, NADP+ as cofactor | Saccharolobus solfataricus | |
0.57 | - |
beta-D-galactose | wild type enzyme, NAD+ as cofactor | Saccharolobus solfataricus | |
1.3 | - |
beta-D-glucose | wild type enzyme, NADP+ as cofactor | Saccharolobus solfataricus | |
1.5 | - |
beta-D-glucose | wild type enzyme, NAD+ as cofactor | Saccharolobus solfataricus | |
20.4 | - |
D-xylose | mutant enzyme T41A, NADP+ as cofactor | Saccharolobus solfataricus | |
24.8 | - |
beta-D-glucose | mutant enzyme T41A, NAD+ as cofactor | Saccharolobus solfataricus | |
29.2 | - |
D-xylose | mutant enzyme T41A, NAD+ as cofactor | Saccharolobus solfataricus | |
33.3 | - |
beta-D-glucose | mutant enzyme T41A, NADP+ as cofactor | Saccharolobus solfataricus | |
59 | - |
beta-D-glucose | mutant enzyme T41V, NADP+ as cofactor | Saccharolobus solfataricus | |
65.8 | - |
D-xylose | mutant enzyme T41V, NADP+ as cofactor | Saccharolobus solfataricus | |
72.5 | - |
beta-D-glucose | mutant enzyme T41V, NAD+ as cofactor | Saccharolobus solfataricus | |
76.3 | - |
D-xylose | mutant enzyme T41V, NAD+ as cofactor | Saccharolobus solfataricus | |
109 | - |
beta-D-galactose | mutant enzyme T41A, NADP+ as cofactor | Saccharolobus solfataricus | |
118 | - |
beta-D-galactose | mutant enzyme T41A, NAD+ as cofactor | Saccharolobus solfataricus | |
175 | - |
beta-D-galactose | mutant enzyme T41V, NADP+ as cofactor | Saccharolobus solfataricus | |
204 | - |
beta-D-galactose | mutant enzyme T41V, NAD+ as cofactor | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
4 * 40000 | Saccharolobus solfataricus |
160000 | - |
- |
Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | O93715 | - |
- |
Purification (Comment) | Organism |
---|---|
gel filtration on Superdex 75 26/60, anion exchange chromatography, and affinity chromatography using Reactive Red-500 dye affinity medium and an NaCl gradient of 0 to 1.5 M | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-galactose + NADP+ | - |
Saccharolobus solfataricus | D-galactono-1,5-lactone + NADPH | - |
? | |
beta-D-glucose + NAD(P)+ | - |
Saccharolobus solfataricus | D-glucono-1,5-lactone + NAD(P)H + H+ | - |
? | |
D-xylose + NAD(P)+ | - |
Saccharolobus solfataricus | D-xylono-1,5-lactone + NAD(P)H + H+ | - |
? | |
L-arabinose + NAD(P)+ | - |
Saccharolobus solfataricus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 40000 | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
Saccharolobus solfataricus |
glucose dehydrogenase | - |
Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
beta-D-glucose | mutant enzyme T41V, NADP+ as cofactor | Saccharolobus solfataricus | |
6 | - |
beta-D-glucose | mutant enzyme T41V, NAD+ as cofactor | Saccharolobus solfataricus | |
7 | - |
D-xylose | mutant enzyme T41V, NADP+ as cofactor | Saccharolobus solfataricus | |
7 | - |
beta-D-galactose | mutant enzyme T41V, NADP+ as cofactor | Saccharolobus solfataricus | |
8 | - |
beta-D-galactose | mutant enzyme T41V, NAD+ as cofactor | Saccharolobus solfataricus | |
12 | - |
D-xylose | mutant enzyme T41V, NAD+ as cofactor | Saccharolobus solfataricus | |
14 | - |
beta-D-glucose | mutant enzyme T41A, NADP+ as cofactor | Saccharolobus solfataricus | |
22 | - |
D-xylose | mutant enzyme T41A, NADP+ as cofactor | Saccharolobus solfataricus | |
22 | - |
beta-D-galactose | mutant enzyme T41A, NADP+ as cofactor | Saccharolobus solfataricus | |
37 | - |
beta-D-galactose | wild type enzyme, NADP+ as cofactor | Saccharolobus solfataricus | |
39 | - |
beta-D-glucose | mutant enzyme T41A, NAD+ as cofactor | Saccharolobus solfataricus | |
44 | - |
D-xylose | wild type enzyme, NADP+ as cofactor | Saccharolobus solfataricus | |
48 | - |
beta-D-glucose | wild type enzyme, NADP+ as cofactor | Saccharolobus solfataricus | |
56 | - |
beta-D-galactose | mutant enzyme T41A, NAD+ as cofactor | Saccharolobus solfataricus | |
61 | - |
D-xylose | wild type enzyme, NAD+ as cofactor | Saccharolobus solfataricus | |
61 | - |
beta-D-galactose | wild type enzyme, NAD+ as cofactor | Saccharolobus solfataricus | |
75 | - |
beta-D-glucose | wild type enzyme, NAD+ as cofactor | Saccharolobus solfataricus | |
81 | - |
D-xylose | mutant enzyme T41A, NAD+ as cofactor | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharolobus solfataricus | |
NADP+ | - |
Saccharolobus solfataricus |