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Literature summary for 1.1.1.47 extracted from

  • Kobayashi, Y.; Horikoshi, K.
    Purification and properties of NAD-dependent D-glucose dehydrogenase produced by alkalophilic Crynebacterium sp. No. 93-1 (1980), Agric. Biol. Chem., 44, 2261-2269.
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Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 1 M, 71% inhibition Corynebacterium sp.
Ag+ 1 mM, 99% inhibition Corynebacterium sp.
Ca2+ 1 mM, 14% inhibition Corynebacterium sp.
Co2+ 1 mM, 74% inhibition Corynebacterium sp.
Cu2+ 1 mM, 14% inhibition Corynebacterium sp.
dithiothreitol 10 mM, 28% inhibition Corynebacterium sp.
Fe2+ 1 mM, 41% inhibition Corynebacterium sp.
Fe3+ 1 mM, 41% inhibition Corynebacterium sp.
Hg2+ 1 mM, complete inhibition Corynebacterium sp.
KCl 1 M, 44% inhibition Corynebacterium sp.
LiCl 1 M, 64% inhibition Corynebacterium sp.
Mn2+ 1 mM, 16% inhibition Corynebacterium sp.
NaCl 1 M, 28% inhibition Corynebacterium sp.
NADH 0.1 mM, 45% inhibition, noncompetitive inhibitorr of D-glucose Corynebacterium sp.
NADP+ 3 mM, 30% inhibition Corynebacterium sp.
NADPH 0.1 mM, 19% inhibition Corynebacterium sp.
PCMB 0.1 mM, complete inhibition Corynebacterium sp.
Zn2+ 1 mM, 8% inhibition Corynebacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
 NAD+ pH 9.8 Corynebacterium sp.
3.2
-
 gentiobiose pH 9.8 Corynebacterium sp.
11
-
 cellobiose pH 9.8 Corynebacterium sp.
51
-
 D-glucose pH 9.8 Corynebacterium sp.
125
-
 D-xylose pH 9.8 Corynebacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 1 * 51000, strain 93-1, SDS-PAGE Corynebacterium sp.
55000
-
 strain 93-1, gel filtration Corynebacterium sp.

Organism

Organism UniProt Comment Textmining
Corynebacterium sp.
-
-
-
Corynebacterium sp. 93-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
streptomycin treatment, ammonium sulfate, DEAE-cellulose, gel filtration, DE 52 cellulose, hydroxyl apatite, gel filtration Corynebacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Corynebacterium sp.

Storage Stability

Storage Stability Organism
-20°C, 20 mM phosphate buffer, pH 7.3, 1 mM 2-mercaptoethanol, 50% v/v glycerol, stable for at least 3 months Corynebacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucose + NAD+ velocities of the reverse reaction at pH 7.0 and 8.0 are 2.2% and 5.9% respectively, of the oxidative reaction Corynebacterium sp. D-glucono-1,5-lactone + NADH + H+
-
 r
beta-D-glucose + NAD+ 5.9% velocity for reverse reaction compared to forward reaction at pH 8.0 Corynebacterium sp. D-glucono-1,5-lactone + NADH + H+
-
 r
beta-D-glucose + NAD+ velocities of the reverse reaction at pH 7.0 and 8.0 are 2.2% and 5.9% respectively, of the oxidative reaction Corynebacterium sp. 93-1 D-glucono-1,5-lactone + NADH + H+
-
 r
beta-D-glucose + NAD+ 5.9% velocity for reverse reaction compared to forward reaction at pH 8.0 Corynebacterium sp. 93-1 D-glucono-1,5-lactone + NADH + H+
-
 r
beta-D-glucose + NADP+ 1% of the activity with NAD+ Corynebacterium sp. D-glucono-1,5-lactone + NADPH + H+
-
 ?
beta-D-glucose + NADP+ 1% of the activity with NAD+ Corynebacterium sp. 93-1 D-glucono-1,5-lactone + NADPH + H+
-
 ?
cellobiose + NAD+ 89% of the activity with D-glucose Corynebacterium sp. ? + NADH
-
 ?
D-fructose + NAD+ 9% of the activity with D-glucose Corynebacterium sp. ? + NADH
-
 ?
D-glucose + NAD+
-
 Corynebacterium sp. D-glucono-1,5-lactone + NADH + H+
-
 r
D-glucose + NAD+
-
 Corynebacterium sp. 93-1 D-glucono-1,5-lactone + NADH + H+
-
 r
D-xylose + NAD+ 44% of the activity with D-glucose Corynebacterium sp. D-xylono-1,5-lactone + NADH + H+
-
 ?
D-xylose + NAD+ 44% of the activity with D-glucose Corynebacterium sp. 93-1 D-xylono-1,5-lactone + NADH + H+
-
 ?
gentiobiose + NAD+ 86% of the activity with D-glucose Corynebacterium sp. ? + NADH
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 51000, strain 93-1, SDS-PAGE Corynebacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 35
-
 Corynebacterium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40 20°C: about 70% of maximal activity, 40°C: about 60% of maximal activity Corynebacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 pH 7.0, 10 min, stable up to Corynebacterium sp.
50
-
 pH 7.0, 10 min, complete inactivation Corynebacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.8
-
 50 mM glycine-NaOH Corynebacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 pH 6.0: about 40% of maximal activity, pH 9.5: about 45% of maximal activity Corynebacterium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 30°C, 30 min, stable Corynebacterium sp.

Cofactor

Cofactor Comment Organism Structure
NAD+ NAD+ is more effective as cofactor than NADP+ on all substrates tested Corynebacterium sp.
NADP+ NAD+ is more effective as cofactor than NADP+ on all substrates tested Corynebacterium sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information
-
 Corynebacterium sp.

pI Value

Organism Comment pI Value Maximum pI Value
Corynebacterium sp. isoelectric focusing, strain 93-1
-
 4.05