Crystallization (Comment) | Organism |
---|---|
- |
Gluconobacter oxydans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Gluconobacter oxydans | |
Hg2+ | - |
Gluconobacter oxydans | |
Ni2+ | - |
Gluconobacter oxydans | |
PCMB | - |
Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
NADP+ | - |
Gluconobacter oxydans | |
5 | - |
beta-D-glucose | - |
Gluconobacter oxydans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
4 * 40000, SDS-PAGE | Gluconobacter oxydans |
153000 | - |
gel filtration | Gluconobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sephadex, Blue-Dextran Sepharose, DEAE-Sephadex, crystallization | Gluconobacter oxydans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
140 | - |
- |
Gluconobacter oxydans |
Storage Stability | Organism |
---|---|
5°C, crystalline preparation, in ammonium sulfate suspension, stable for several months | Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + NADP+ | in the reverse reaction only 4% of the reaction occurs at neutral pH | Gluconobacter oxydans | D-glucono-1,5-lactone + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 40000, SDS-PAGE | Gluconobacter oxydans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | higher reaction rate in Tris-HCl buffer compared to potassium phosphate buffer | Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | completely inactive with NAD+ | Gluconobacter oxydans | |
NADP+ | enzyme is specific for NADP+ and completely inactive with NAD+ | Gluconobacter oxydans |